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Overexpression and extra-mitochondrial localization of the chaperonin Hsp60 in ameloblastoma
Institution:1. Departmento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico;2. División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Unidad Xochimilco, Mexico City, Mexico;3. Departamento de Patología y Medicina Bucal, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City, Mexico;4. Departamento de Cirugía Maxilofacial, Hospital Juárez de México Mexico City, Mexico;1. Department of Endodontics, The Nippon Dental University School of Life Dentistry at Tokyo, Tokyo, Japan;2. Department of Microbiology, The Nippon Dental University School of Life Dentistry at Tokyo, Tokyo, Japan;3. Research Center for Odontology, The Nippon Dental University School of Life Dentistry at Tokyo, Tokyo, Japan;1. Division of Physiology, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan;2. Section of Primary Dental Education, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan;3. Division of Oral and Maxillofacial Radiology, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan;1. Department of Orthodontics, Matsumoto Dental University, Shiojiri, Nagano, Japan;2. Division of Oral and Maxillofacial Biology, Institute for Oral Science, Matsumoto Dental University, Shiojiri, Nagano, Japan;3. Department of Oral and Maxillofacial Biology, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri, Nagano, Japan;4. Department of Prosthodontics, Matsumoto Dental University, Shiojiri, Nagano, Japan;5. Department of Oral Physiology, Graduate School of Dentistry, Osaka University, Suita, Japan;1. Department of Periodontology, Faculty of Dentistry, Hacettepe University, Ankara, Turkey;2. Department of Physics Engineering, Hacettepe University, Ankara, Turkey;3. Department of Anatomy, Hacettepe University Faculty of Medicine, Ankara, Turkey;4. Department of Biostatistics, Faculty of Medicine, Hacettepe University, Ankara, Turkey;5. Department of Prosthodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey;1. Department of Anatomy, Tokyo Dental College, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;2. Tokyo Dental College Research Branding Project, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;3. Department of Fixed Prosthodontics, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;4. Department of Oral Health and Clinical Science, Division of Dysphagia Rehabilitation, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;5. Laboratory of Biology, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;1. Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;2. Gerontology, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;3. Oral Functional Anatomy, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;4. Periodontology and Endodontology, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;5. Orthodontics, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;6. Oral Functional Prosthodontics, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;7. Northern Army Medical Unit, Camp Makomanai, Japan Ground Self-Defense Forces, Sapporo, Japan;8. Shandong Provincial Key Laboratory of Oral Biomedicine, The School of Stomatology, Shandong University, Jinan, China
Abstract:ObjectivesAmeloblastoma is an odontogenic neoplasm of the mandible and maxilla with various histological types and subtypes. It has been reported that some ameloblastomas could arise from dentigerous cyst walls; thus, the development of ameloblastoma from dentigerous cysts may be due to differential protein expression. Our aim was to identify a membrane protein that is differentially expressed in ameloblastomas with respect to dentigerous cysts.MethodsWe analyzed the SDS-PAGE profiles of membrane proteins from ameloblastomas and dentigerous cysts. The protein in a band present in the ameloblastoma sample, but apparently absent in the dentigerous cyst sample was identified via mass spectrometry as the chaperonin Hsp60. We used western blotting and immunohistochemistry to analyze its overexpression and localization in ameloblastoma.ResultsWe found a differential band of 95 kDa in the membrane proteins of ameloblastoma. In this band, the chaperonin Hsp60 was identified, and its overexpression was corroborated using western blotting and immunohistochemistry. Hsp60 was localized in the plasma membrane of all ameloblastoma samples studied; in addition, it was found in the cell nucleus of the plexiform subtype of conventional ameloblastoma.ConclusionsOur results suggest that Hsp60 may be involved in ameloblastoma development, and could therefore be a potential therapeutic target for ameloblastoma treatment.
Keywords:Dentigerous cyst  Differential expression  Membrane proteins  Odontogenic tumors  Hsp60  DC"}  {"#name":"keyword"  "$":{"id":"kwrd0040"}  "$$":[{"#name":"text"  "_":"dentigerous cyst  AM"}  {"#name":"keyword"  "$":{"id":"kwrd0050"}  "$$":[{"#name":"text"  "_":"conventional ameloblastoma  FAM"}  {"#name":"keyword"  "$":{"id":"kwrd0060"}  "$$":[{"#name":"text"  "_":"follicular ameloblastoma  PAM"}  {"#name":"keyword"  "$":{"id":"kwrd0070"}  "$$":[{"#name":"text"  "_":"plexiform ameloblastoma  UA"}  {"#name":"keyword"  "$":{"id":"kwrd0080"}  "$$":[{"#name":"text"  "_":"unicystic ameloblastom  LUA"}  {"#name":"keyword"  "$":{"id":"kwrd0090"}  "$$":[{"#name":"text"  "_":"luminal unicystic ameloblastoma  MUA"}  {"#name":"keyword"  "$":{"id":"kwrd0100"}  "$$":[{"#name":"text"  "_":"mural unicystic ameloblastoma
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