Application of hiPSCs in tooth regeneration via cellular modulation |
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| Affiliation: | 1. Department of Oral Biochemistry, Division of Oral Structure, Function and Development, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu, 501-0296, Japan;2. Chemistry Laboratory, Department of Business Administration, Asahi University School of Business Administration, 1851-1 Hozumi, Mizuho, Gifu, 501-0296, Japan;3. Department of Oral and Maxillofacial Surgery, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu, 501-0296, Japan;4. Department of Oral Biochemistry, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu, 501-0296, Japan;1. Division of Physiology, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan;2. Section of Primary Dental Education, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan;3. Division of Oral and Maxillofacial Radiology, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan;1. Department of Zoology, Guru Ghasidas Vishwavidyalaya, Bilaspur, India;2. Department of Biochemistry, Late Atal Bihari Vajpayee Memorial Medical College Rajnandgaon (C.G.), India;3. Department of Public Health, Nellore Municipal Corporation, Nellore, India;4. Department of Immunopathology, Institute of Lungs Biology and Disease, Comprehensive Pneumology Center, Helmholtz Zentrum, Neuherberg, Munich, 85764, Germany;1. Department of Orthodontics, Matsumoto Dental University, Shiojiri, Nagano, Japan;2. Division of Oral and Maxillofacial Biology, Institute for Oral Science, Matsumoto Dental University, Shiojiri, Nagano, Japan;3. Department of Oral and Maxillofacial Biology, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri, Nagano, Japan;4. Department of Prosthodontics, Matsumoto Dental University, Shiojiri, Nagano, Japan;5. Department of Oral Physiology, Graduate School of Dentistry, Osaka University, Suita, Japan;1. Department of Anatomy, Tokyo Dental College, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;2. Tokyo Dental College Research Branding Project, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;3. Department of Fixed Prosthodontics, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;4. Department of Oral Health and Clinical Science, Division of Dysphagia Rehabilitation, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;5. Laboratory of Biology, 2-9-18 Kanda-misakicho, Tokyo, 101-0061, Japan;1. Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;2. Gerontology, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;3. Oral Functional Anatomy, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;4. Periodontology and Endodontology, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;5. Orthodontics, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;6. Oral Functional Prosthodontics, Graduate School of Dental Medicine, Faculty of Dental Medicine, Hokkaido University, Sapporo, Japan;7. Northern Army Medical Unit, Camp Makomanai, Japan Ground Self-Defense Forces, Sapporo, Japan;8. Shandong Provincial Key Laboratory of Oral Biomedicine, The School of Stomatology, Shandong University, Jinan, China |
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| Abstract: | BackgroundInduced pluripotent stem cell (iPSC)-based technology provides limitless resources for customized development of organs without any ethical concerns. In theory, iPSCs generated from terminally differentiated cells can be induced to further differentiate into all types of organs that are derived from the embryonic germ layers. Since iPSC reprogramming technology is relatively new, extensive efforts by the researchers have been put together to optimize the protocols to establish in vitro differentiation of human iPSCs (hiPSCs) into various desirable cell types/organs.HighlightsIn the present study, we review the potential application of iPSCs as an efficient alternative to primary cells for modulating signal molecules. Furthermore, an efficient culture system that promotes the differentiation of cell lineages and tissue formation has been reviewed. We also summarize the recent studies wherein tissue engineering of the three germ layers has been explored. Particularly, we focus on the current research strategies for iPSC-based tooth regeneration via molecular modulation.ConclusionIn recent decades, robust knowledge regarding the hiPSC-based regenerative therapy has been accumulated, especially focusing on cellular modulation. This review provides the optimization of the procedures designed to regenerate specific organs. |
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