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Metformin pre-conditioning enhances the angiogenic ability of the secretome of dental pulp stem cells |
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| Authors: | Nezar Boreak Noor Mohammed Abu Khayrat Ahmed Omar Shami Haitham Jameel Mohammed Zaylaee Aeshah Ali Hanbashi Sara Abdulhamed Souri Hala Mohammed Otayf Rafif Essam Bakri Mohammad Eissa Mansoor Ajeely Alwaleed Essam Hassan Bakri Mohammed Abdurabu Jafer A Thirumal Raj Hosam Ali Baeshen Shankargouda Patil |
| Institution: | 1. Department of Restorative Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia;2. College of Dentistry, Jazan University, Jazan, Saudi Arabia;3. Department of Preventive Dental Science, College of Dentistry, Jazan University, Saudi Arabia;4. Department of Health Promotion, Maastricht University/CAPHRI, The Netherlands;5. Department of Oral Pathology and Microbiology, Sri Venkateswara Dental College and Hospital, Chennai, India;6. Department of Orthodontics, College of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia;7. Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Saudi Arabia |
| Abstract: | The aim of the present study was to assess the influence of metformin on the angiogenic ability of secretomes from dental pulp stem cells. The stem cells were obtained from the dental pulp (DPSCs) (n = 3) using the explant culture method. We treated the DPSCs with different concentrations of metformin and assessed the expression of the angiogenesis-related genes. We also tested the angiogenic effect of the secretomes on the yolk sac membrane of the chick embryos by counting the quaternary blood vessel formations on the yolk sac membrane. We found that metformin treatment enhanced the angiogenic potential of the stem cell secretome in a dose-dependent manner. This was evidenced by the increase in the quaternary blood vessel formations in the yolk sac membrane with lower to higher concentrations of metformin. Pre-treatment with metformin modulates the angiogenic potential of the stem cell-conditioned media in a dose-dependent manner. The augmentation of the angiogenic potential of the DPSCs can aid regeneration, especially in scenarios requiring the regeneration of vacuoles. |
| Keywords: | Angiogenesis Dental pulp Metformin Secretome Stem cells |
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