湖南籍汉族正常人及肺结核病人人类白细胞抗原-DQCAR微卫星多态性分析

目的 研究HLA－DQCAR微卫星DNA在湖南籍汉族正常人及肺结核病人中的分布，探讨该基因与湖南籍汉族肺结核病的相关性。方法 用DQCAR特异性引物扩增该微卫星片段，对扩增片段行变性聚丙烯酰胺凝胶电泳来分析其多态性，并经测序确定片段长度。结果 调查湖南籍汉族健康人68例，发现DQCAR等位基因10个，分别为DQCAR101、103、105、107、111、113、115、117、119、121，各等位基因在人群中的分布不均匀，以DQCAR113、DQCAR115分布较为集中（基因频率分别为31．4％、22．4％），与美国、韩国人群中的基因频率比较，发现该基因分布具有较大的种族差异性。调查湖南籍汉族肺结核患者79例，发现等位基因12种，除上述等位基因外，还有DQCAR99和109，并发现DQCAR115在肺结核患者中显著增高（基因频率35．4％，RR＝2．12，Pc＜0．05）。结论 DQCAR微卫星各等位基因在湖南汉族人群中的分布具有多态性；DQCAR115与湖南籍汉族肺结核病相关。

Investigation of the polymorphism of HLA-DQCAR microsatellites in the healthy and pulmonary tuberculosis in Hunan Hans

Objective To observe HLA DQCAR microsatellite distribution and the association of this gene with pulmonary tuberculosis in Hunan Hans. Methods The polymorphism of HLA DQCAR microsatellite was analyzed by electrophoresis on a 8% denaturing polyacrylamide gel The length of its alleles was determined by sequencing Results Ten alleles,DQCAR101,103,105,107,111,113, 115,117,119 and 121,were found in 68 Hunan healthy Hans,but the frequencies of them were different In addition to the above mentioned alleles, DQCAR 99 and 109, were also found in 79 pulmonary tuberculosis patients in Hunan Hans This results as well showed that DQCAR115 allele was significantly associated with pulmonary tuberculosis in Hunan Hans(RR=2 12, Pc <0 05) Conclusions The frequency of DQCAR alleles is of great polymorphism DQCAR115 is significantly associated with pulmonary tuberculosis in Hunan Hans