瘦素对肝细胞三酰甘油沉积的影响

目的观察瘦素对人L-02脂肪变肝细胞三酰甘油(TG)沉积的影响,探讨瘦素在非乙醇性脂肪性肝病发生、发展中的作用。方法人肝L-02细胞培养接种于50 m l培养瓶中,用50%胎牛血清1640培养基造模,时间24 h。设立正常组、模型组、瘦素处理1,2,3组,处理组瘦素浓度分别为10-7mol/L,10-6mol/L,10-5mol/L,分别处理6 h;再以10-6mol/L浓度瘦素建立时效关系分别处理(6,12及24 h)。每组均行油红“O”染色观察脂肪化情况,高效液相色谱测细胞内TG含量变化。结果油红“O”染色显示50%的胎牛血清的1640培养基24 h能成功地制造肝细胞脂肪变性模型,加入瘦素处理后脂肪化程度减轻。高效液相色谱显示细胞内TG明显减少,且与瘦素呈剂量、时间依赖性关系。结论肝细胞脂肪变性时,瘦素能使肝细胞脂肪化程度减轻,能减少肝细胞TG的沉积,对脂肪肝防治有一定的作用。

Effects of Leptin on Disposition of Triglyceride in Fatty Liver Cells

Objective To explore the effect of leptin on triglyceride's disposition in normal and nonalcoholic induced fatty liver cell.Methods L-02 liver cells were cultured in 50 ml culture dish.Fatty liver cell models were made with 50% inactivated embryo blood serum for 24 hours.Results Oil red staining showed that 50% inactivated embryo blood serum can make fatty liver cell model.After L-02 liver cells were turned into fatty liver cells,leptin was added to fatty liver cells.The HPLC showed that the model group's triglyceride disposition increased clearly,but after leptin was given into the model cells,triglyceride's disposition decreased obviously, which was positive correlated with leptin's concentration.Conclusion When liver L-02 cells were turned into fatty liver cell,leptin can inhibit triglyceride's disposition.Leptin has some therapeutic effect on fatty liver cells.