HPV16-E6蛋白在宫颈病变组织中的表达及临床应用

目的 研究人乳头瘤病毒16型E6蛋白（human papillomavirus-16 E6 protein,HPV16-E6）在宫颈病变组织中的表达及其与临床病理特征之间的关系.方法 采用免疫组化S-P法检测HPV16-E6蛋白在宫颈正常组织、慢性炎症、宫颈上皮内瘤变（cervical intraepithelial neoplasia,CIN）、浸润癌上皮中的阳性表达情况.结果 HPV16-E6在正常宫颈、宫颈炎、CIN,宫颈癌各组中,阳性表达率分别为0.5%、38.35%、46.15%、71.11%,4组比较差异有统计学意义（χ2=27.50,P〈0.01）.经χ2分割后比较（α′=0.007143）,宫颈癌组、CIN组、宫颈炎组的阳性率显著高于正常宫颈组（P〈0.05）,宫颈癌组的阳性率显著高于宫颈炎组（P〈0.01）.HPV16-E6与年龄、病理类型、宫旁或脉管浸润相关（P〈0.05）.结论 HPV16-E6与宫颈癌的启动、发生、发展及侵袭转移均密切相关.HPV疫苗对于宫颈癌的防治有广泛的应用前景.

The expression and clinical significance of HPV16-E6 in the pathological tissues of cervix

Objective To investigate the relationship between the expression of human papillomavirus-16 E6 protein （HPV16-E6） and clinical pathological characteristics. Methods The expression of HPV16-E6 protein in patients with cervical cancer, cervical intraepithelial neoplasia （CIN）, chronic cervieitis and normal cervical epithelium were examined by immunohistochemitry. Results The positive rate of HPV16-E6 protein was 71.11% ,46.15%, 38.35%, 0.5% in cervical cancer, C IN, chronic cervicitis and normal cervical epithelium respectively. There were significant differences in the positive rates among the 4 groups （ P 〈 0.01 ）. The positive rate in cervical cancer group was significantly higher than that in chronic cervicitis group and normal cervical epithelium group（ P 〈 0.01 ）. The positive rate in CIN and cervicitis group was significantly higher than that normal cervical epithelium group（ P 〈0.01 ）. The positive expression of HPV16 -E6 was correlated with the age of the patients , pathological types and the infiltration of latero - uterus or haemal tube （ P 〈 0.05 ）. Conclusion HPV16-E6 plays an important role in the genesis ,development,invasion and metastasis of cervical carcinoma. The vaccine of HPV has wide application prospect in the prevention and treatment of cervical carcinoma.