Mobilization and evolutionary history of miniature inverted-repeat transposable elements (MITEs) in Beta vulgaris L. |
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Authors: | Gerhard Menzel Daryna Dechyeva Heiko Keller Cornelia Lange Heinz Himmelbauer Thomas Schmidt |
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Affiliation: | (1) Institute of Botany, Dresden University of Technology, D-01062 Dresden, Germany;(2) Max-Planck-Institute for Molecular Genetics, D-14195 Berlin, Germany |
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Abstract: | We have identified three families of miniature inverted-repeat transposable elements (VulMITEs) in the genome of sugar beet
(Beta vulgaris L.), evidently derived from a member of the Vulmar family of mariner transposons. While VulMITEs I are typical stowaway-like MITEs, VulMITEs II and VulMITEs III are rearranged stowaway elements of increased size. The integration of divergent moderately and highly repetitive sequences into VulMITEs II and,
in particular in VulMITEs III, respectively, shows that amplification of repetitive DNA by MITEs contribute to the increase
of genome size with possible implications for plant genome evolution. Fluorescent in-situ hybridization (FISH), for the first time visualizing stowaway MITE distribution on plant chromosomes, revealed a dispersed localization of VulMITEs along all B. vulgaris chromosomes. Analysis of the flanking sequences identified a dispersed repeat as target site for the integration of the stowaway element VulMITE I. Recent transposition of VulMITE I, which most likely occurred during the domestication of cultivated beets, was concluded from insertional polymorphisms between
different B. vulgaris cultivars and species.
†Sequence data from this article have been deposited in the EMBL/GenBank Data Library under the accession nos. AM231630-AM231653
and AM259123-AM259125. |
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Keywords: | Beta vulgaris mariner transposon miniature inverted repeat stowaway Vulmar1 |
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