骨髓增生异常综合征患者骨髓VEGF和bFGF水平的变化及其意义

目的:探讨骨髓增生异常综合征(MDS)患者骨髓浆液中血管内皮细胞生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)水平变化及其临床意义。方法:采用酶联免疫吸附法(ELISA法)检测38例MDS、10例急性非淋巴细胞白血病(ANLL)患者和15例正常人骨髓浆液的VEGF和bFGF水平,用酶标仪测定VEGF及bFGF光密度平均值。结果:MDS组和ANLL组骨髓浆液VEGF和bFGF水平均显著高于正常对照组(P<0.05,P<0.01),而MDS组VEGF水平显著低于ANLL组(P<0.05),但MDS组bFGF水平与ANLL组之间差异无统计学意义(P>0.05)。骨髓浆液VEGF和bFGF表达与MDS患者性别、年龄、外周血中白细胞、血小板及血红蛋白含量及骨髓涂片中原始细胞百分比(高危组)均无相关(r分别为0.160、0.154、0.193、0.185、0.171、0.286;r分别为0.141、0.175、0.152、0.163、0.158、0.239,P均>0.05)。结论:MDS患者骨髓微环境中bFGF和VEGF表达水平均升高,可能在MDS骨髓血管新生及其病情发展过程中发挥了重要作用。

Clinical Significance of the Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Expression in the Patients with Myelodysplastic Syndromes

Objective:To investigate the vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF) levels in bone marrow and their clinical significance in patients with myelodysplastic syndromes(MDS).Methods:The VEGF and bFGF concentrations were measured using an enzyme-linked immunosorbent assay in bone marrow samples of 38 cases of MDS and 30 cases of the control which included 15 cases of acute non-lymphocytic leukemia(ANLL),15 cases of normal volunteers.Results:The VEGF and bFGF levels in bone marrow fluid of patients with MDS and ANLL were higher than that in normal controls(P<0.05),and the VEGF level in MDS group was lower than that in ANLL group(P<0.05).But there was no difference between the bFGF level in MDS group and that in AML group(P>0.05).There was no correlation between VEGF and bFGF levels and the percentage of blasts in the bone marrow of patients with high-risk MDS.Conclusion:VEGF and bFGF may play a role in the angiogenesis and pathophysiology of MDS and anti-angiogenesis could constitute a novel strategy for the treatment of MDS.