阿糖胞苷对白血病飚62细胞增殖和凋亡的影响

目的探讨阿糖胞苷对诱导入白血病细胞株K562细胞增殖和凋亡的影响。方法采用噻唑蓝比色法（MTT）检测不同剂量的阿糖胞苷作用于K562细胞24h、48h、72h增殖影响，流式细胞术检测细胞周期及凋亡率。结果各浓度阿糖胞苷对K562细胞的生长具有明显抑制作用。在同一浓度下，抑制增殖作用随阿糖胞苷作用时间的延长而增强，呈现出剂量和时间效应关系。阿糖胞苷作用于K562细胞后，G0／G-期细胞所占比例随着阿糖胞苷作用时间的延长而增加，S期与G2／M期细胞所占比例则呈时间依赖性下降。阿糖胞苷作用于K562细胞后，凋亡的K562细胞所占比例随着药物浓度和作用时间的增加而增加，呈现对阿糖胞苷的时间．浓度依赖性。结论阿糖胞苷对人自血病细胞株K562细胞的增殖有明显的抑制作用，并可以时间和浓度依赖方式促进K562细胞的凋亡。

Influence of cytarabine on proliferation and Apoptosis of K562 cells

Objective To explore the mechanisms of proliferation and apoptosis of K562 cells induced by cytarabine. Methods Different doses of cytarabine on the proliferotion at K562 cell 24h,48h,72h were detected by MTT. Cell cycle And Apoptosis rate were detected by FCM. Results Each concentration of cytarabine obvious inhibited the growth of K562 cells. In the same concentrations,function of proliferation inhibition with cytarabine increased by the extension of time,which showed dose and time effect relations. Cytarabine on K562 cell,G0/G1 phase cell proportion of role as cytarabine increased when extension of time, S period and the G2/M period cells were decreased.Apoptosis of K562 cell in proportion as drug concentration and effect time increases, which present Cytarabine timeconcentration dependence. Condusion Cytarabine was apparent inhibition to proliferation of K562 cell leukemia cell, which could promote K562 cell apoptosis by time and concentration dependence way.