| 温郁金二萜类化合物C对人胃癌SGC-7901细胞中Caspase-9,3,7和PARP(89KD)蛋白表达的影响 |
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| 引用本文： | 金海峰,吕宾,陈喆,马忠俊,王楼蕾.温郁金二萜类化合物C对人胃癌SGC-7901细胞中Caspase-9,3,7和PARP(89KD)蛋白表达的影响[J].中华中医药杂志,2011(2):395-398. |
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| 作者姓名： | 金海峰 吕宾 陈喆 马忠俊 王楼蕾 |
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| 作者单位： | 浙江中医药大学附属第一医院消化科;浙江大学药学院现代中药研究所;浙江大学医学院附属第一医院传染科; |
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| 摘 要： | 目的:探讨温郁金二萜类化合物C1]对人胃癌SGC-7901细胞中Caspase-9,Caspase-3,Caspase-7和PARP(89KD)蛋白表达的影响。方法:用从温郁金醚提物中提取得到的二萜类化合物C分别以0、40、55、70μg/mL 4个浓度作用于人胃癌SGC-7901细胞24h;用Western Blot杂交法检测4个浓度组中人胃癌SGC-7901细胞的Caspase-9、Caspase-3、Caspase-7和PARP(89KD)蛋白量的表达。结果:温郁金二萜类化合物C能上调SGC-7901细胞中Caspase-9、Caspase-3、Caspase-7及PARP(89KD)蛋白的表达,与空白对照组相比,差异具有统计学意义(P<0.05);且具有一定的剂量依赖性。结论:温郁金二萜类化合物C可通过上调Caspase-9,Caspase-3,Caspase-7,PARP(89KD)的表达来诱导人胃癌SGC-7901细胞发生凋亡。
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| 关 键 词： | 温郁金 二萜类化合物C 胃癌 凋亡 |
Influence of diterpenoid curcumrinol C from ether extract of Radix Curcumae on human gastric cancer cell SGC-7901 in expression of Caspase-9,3,7,and PARP(89KD) |
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| JIN Hai-feng,LV Bin,CHEN Zhe,MA Zhong-jun,WANG Lou-Lei.Influence of diterpenoid curcumrinol C from ether extract of Radix Curcumae on human gastric cancer cell SGC-7901 in expression of Caspase-9,3,7,and PARP(89KD)[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(2):395-398. |
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| Authors: | JIN Hai-feng LV Bin CHEN Zhe MA Zhong-jun WANG Lou-Lei |
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| Institution: | JIN Hai-feng1,LV Bin1,CHEN Zhe1,MA Zhong-jun2,WANG Lou-Lei3(1The First Affiliated Hospital,Zhejiang Chinese Medical University,Hangzhou 310006,China,2College of Pharmacy,Zhejiang University,Hangzhou310058,3The First Affiliated Hospital of Medical School of Zhejiang University,Hangzhou 310003,China) |
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| Abstract: | Objective: To investigate the effect on expression of Caspase-9,3,7,and PARP(89KD) of SGC-7901 by diterpenoid curcumrinol C from ether extract of Radix Curcumae.Methods: SGC-7901 cells were treated by curcumrinol C on four different concentrations(0,40,55,70μg/mL) and Western Blot hybridization was used for detecting expression of Caspase-9,3,7 and PARP(89KD) of SGC-7901 cells after treated by curcumrinol C.Results: Compared with the control group,curcumrinol C could increased the expression of Caspase-9,Caspase-3,Caspase-7 and PARP(89KD)(P<0.05),which showed a dose-dependent.Conclusion: Curcumrinol C can induce tumor cells apoptosis by increasing the expression of Caspase-9,Caspase-3,Caspase-7 and PARP(89KD). |
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| Keywords: | Radix Curcumae Diterpenoid curcamrinaloc Gastric carcinoma Apoptosis |
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