补肾中药天癸方对雄激素致不孕大鼠胰腺及卵巢雄激素受体的影响

目的:探讨补肾中药天癸方对雄激素致不孕大鼠(ASR)促排卵的作用机制。方法:新生SD雌性大鼠随机分为3组,ASR模型组:9d龄SD雌性大鼠皮下注射丙酸睾丸酮;中药组:ASR模型在81d龄起每日灌服天癸方;对照组:9d龄SD雌性大鼠皮下注射中性茶油。在106d龄左右(动情前期),放免法测定血Δ4-雄烯二酮(Δ4-A)、总睾酮(TT)、游离睾酮(FT)、雌二醇(E2)、胰岛素(Ins)和C-肽(C-P),RT-PCR方法检测胰腺及卵巢雄激素受体(AR)mRNA表达水平,并观察中药治疗前后上述指标的变化。结果:ASR模型血Δ4-A、TT、FT、E2、Ins、C-P均显著高于对照组(P<0.05,P<0.01),胰腺、卵巢的AR mRNA表达水平明显升高(P<0.01);中药天癸方治疗后,排卵率为58%,各项血激素及胰腺AR mRNA表达水平明显下降,与模型组比较有显著性差异(P<0.05,P<0.01),卵巢AR mRNA水平与治疗前相比无明显改变。结论:ASR模型血雄激素水平升高,上调胰腺、卵巢的AR mRNA表达,引起高胰岛素血症和无排卵。天癸方可降低血雄激素和胰岛素,使胰腺AR mRNA表达水平下降,纠正ASR模型的内分泌-代谢失调状态,虽然卵巢的AR mRNA表达水平仍较高,但在正常的激素环境下仍出现了排卵。

Effect of tonifying kidney herbs Tiangui Recipe on androgen receptor levels of ovary and pancreas in androgen sterilized rats

Objective:To investigate the mechanism of inducing ovulation with kidney tonifying herbs Tiangui Recipe in androgen sterilized rats(ASR).Methods:The experiment new born SD female rats were divided randomly into 3 groups:the ASR model group,the SD rats were injected with testosterone propionate subcutaneous at the age of 9 days;the herb group,ASR were administered with Tian Gui Fang daily at the age of 80 days for two weeks;the control group,SD rats were injected with tea oil subcutenously at the age of 9 da...