大鼠海马神经元白细胞介素2的变化与癫痫发生的相关性

背景中枢神经系统中的海马逐渐成为近来研究癫痫发病的重要区域,细胞因子中的白细胞介素2(interleukin-2,IL-2)是一种重要的免疫因子,同时又是中枢神经系统中的一种重要的神经调质.在体及离体的研究证实脑内存在IL-2及IL-2R,IL-2能影响下丘脑-垂体轴的功能,并能引起行为及脑电图的改变,IL-2与癫痫发病的联系得到广大学者的关注.目的探讨细胞因子中IL-2与癫痫发病之间的关系.设计随机对照的实验研究.单位江汉大学医学与生命科学学院,华中科技大学同济医学院,中国科学院武汉物理与数学研究所波谱与原子分子国家重点实验室.材料实验于2002-01/12在华中科技大学同济医学院脑研究室完成.健康成年Wistar大鼠52只,雌雄不限,体质量200～250 g,实验前均饲养于安静、无强光刺激的环境中.方法将Wistar大鼠随机分成3组,实验组侧脑室注入马桑内酯(coriari lactone,CL)2 μL,生理盐水组注入等量的生理盐水,空白对照组不做任何处理.然后运用免疫组织化学方法,对大鼠海马IL-2的免疫反应性进行分析;同时对实验中大鼠的行为及脑电图进行观察和记录.结果实验组大鼠侧脑室注射CL过程中即诱发癫痫,且测到痫性脑电图,生理盐水组和空白对照组无癫痫症状且未记录到痫性脑电图;实验组大鼠海马IL-2免疫反应性较生理盐水组和空白对照组显著增强(P＜0.01),表现在海马CA1,CA2,CA3区细胞数目增多,突起及其分支增多,着色明显加深;而生理盐水组和空白对照组之间无明显差别(P＞0.05).结论IL-2参与癫痫的发病过程,二者之间存在着一定的联系.

Correlation between interleukin-2 in rat hippocampal neurons and the onset of epilepsy

BACKGROUND: The hippocampus of the central nervous system(CNS) has been recognized as an important region in relation to epileptic seizure. Interleukin-2(IL-2) is a key immune factor and also an important neuromodulator of CNS. The researches in vivo and in vitro show that both IL-2and its soluble receptor(IL-2R) exist in the brain, and IL-2 can influence the function of the hypothalamus-hypophysis axis and cause the changes in behavior and electroencephalogram(EEG) . Currently much attention has been given to the relationship between the onset of epilepsy and IL-2.OBJECTIVE: To investigate the relationship between IL-2 and the onset of epilepsy in the cytokine.DESIGN: A randomized and controlled trial.SETTING: College of Medical and Life Sciences, Jianghan University;Tongji Medical College, Huazhong University of Science and Technology;National Key Laboratory of Spectroscopy of Atomic and Molecular, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences.MATERIALS: The experiment was conducted in the Laboratory of Brain,Tongji Medical College, Huazhung University of Science and Technology from January to December 2002. Fifty-two healthy adult Wistar rats of either sex weighing 200- 250 g were provided by the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology. All rats were fed in a quiet environment without any strong photostimulation.METHODS: The rats were randomly divided into three groups to receive the injection of 2 μL coriaria lactone(CL) into the lateral cerebral ventricle(experimental group), injection of saline of equivalent volume (saline group)and no treatment(blank control group), respectively. Immunohistochemical method was used to analyze the immunoreactivity of the rat hippocampus,and the behavioral and EEG changes were recorded.RESULTS: Epilepsy was induced even before the completion of the injection of CL into lateral cerebral ventricle in the experimental group, and epileptic EEG was detected. Epileptic symptom or EEG was not observed in the saline group and blank control group. IL-2 immunorecativity of the hippocampus in the experimental group was much stronger than that of the other two groups( P＜ 0. 01), shown by increased amount of cells in the hippocampal CA1, CA2and CA3 regions and the increased processes and branches of the cells, with obviously intensified the staining. However, there were no significant changes both in saline group and blank control group( P ＞ 0.05).CONCLUSION: IL-2 participates in the pathogenesis of epileptic seizure with a certain correlation.