水飞蓟宾磷脂复合物对大鼠自身免疫性前列腺炎炎性细胞因子的影响

目的：研究水飞蓟宾磷脂复合物（SPC）对大鼠自身免疫性前列腺炎炎性细胞因子的影响。方法：将大鼠随机分为正常对照组、模型组、普适泰26mg／（kg·d）]组和高、中、低剂量84、42、21mg／（kg·d）]SPC组，每组8只。除正常对照组外，其余各组采用免疫佐剂法建立大鼠自身免疫性前列腺炎模型，灌胃给予相应药物，连续给药30d。检测各组大鼠末次给药24h后前列腺、脾、胸腺的脏器指数，前列腺组织病理变化，血清中白细胞介素8（IL-8）、IL-10、肿瘤坏死因子a（TNF-α）的水平。结果：各组大鼠的脏器指数差异均无统计学意义（P〉O．05）。与正常对照组比较，模型组大鼠的前列腺组织有明显炎症反应，血清中IL-8、IL-10、TNF-α水平均明显增加（P〈0．05）；与模型组比较，普适泰组和中、高剂量SPC组大鼠前列腺组织的炎症反应均有好转，血清中IL-8、IL-10、TNF-α水平均明显减少（P〈0．05），低剂量SPC组大鼠血清中仅TNF-α水平明显减少（P〈0．05），其余指标间差异无统计学意义（P〉O．05）。结论：SPC能显著改善自身免疫性前列腺炎模型大鼠的免疫功能，并具有减轻前列腺免疫炎症反应的作用。

Effects of Silybin-phospholipid Complex on Inflammatory Factors ofAutoimmune Prostatitis in Rats

OBJECTIVE： To explore the effects of Silybin-phosphlipid complex （SPC） on inflammatory factor of autoimmune prostatitis in rats, METHODS： The experimental rats were randomly divided into normal control group, model group, Pushitai group 26 mg/（kg, d）] and high-dose, medium-dose and low-dose 84, 42 and 21 mg/（kg, d）] SPC groups with 8 rats in each group. Autoimmune prostatitis model was established in those group except normal control group, and then given relevant medicine intragastrically for 30 days. The prostate tissue, spleen and thymus gland index, and the pathologic changes of prostatic tissue were determined, and the expressions of IL-8, IL-10 and TNF-α were also detected 24 h after the last medication. RESULTS： There were no significant difference in organ index of each group （P〉0.05）. Compared with normal control group, significant inflamma- tory reaction was found in prostatic tissue, and the levels of IL-8, IL-10, TNF-ct in serum of model group were increased signifi- cantly （P〈0.05）. Compared with model group, inflammatory reaction of prostatic tissue was recovered in Pushitai group and medi- um-dose and high-dose SPC groups, while the levels of IL-8, IL-10 and TNF-α in serum were decreased obviously （P〈0.05）, just the level of TNF-α in serum of low-dose SPC group was decreased obviously （P〈0.05）, the other index had no significant dif- ference （P〉0.05）. CONCLUSIONS： SPC can improve immune function of autoimmune prostatitis rat and alleviate the prostatic immune inflammatory responses.