钼对人食管癌细胞ECA-109的化疗增敏作用及对食管癌干细胞p75NTR的影响

目的:探讨钼对食管癌细胞ECA-109化疗敏感性的影响及对食管癌干细胞p75NTR作用.方法:本实验选用人食管癌细胞(esophageal cancer cells,ECCs)ECA-109.设计分为4组:空白对照组、顺铂组、单纯加钼组、顺铂加钼组,后3组均采用不同的浓度进行试验.用MTT法检测各组对人食管癌细胞ECA-109的生长抑制作用;流式细胞仪检测各组p75NTR百分率的变化.结果:顺铂组各浓度对食管癌细胞ECA-109有一定的抑制作用,且对食管癌干细胞也有不同程度的抑制作用,并且随着给药浓度和时间的增加均呈增强趋势;单纯加钼组对食管癌细胞ECA-109和对食管癌干细胞的抑制作用均不明显;顺铂加钼组对食管癌细胞ECA-109和食管癌干细胞的抑制作用则明显增强,与单用同浓度顺铂组及空白对照组比较有差异性(P<0.05),且呈一定的浓度、时间依赖性.结论:钼可明显增强顺铂对食管癌细胞ECA-109和食管癌干细胞的抑制作用,而单用钼则达不到理想的效果,说明钼可作为化疗增敏剂,为其作为食管癌化疗的辅助剂提供了实验依据.

Molybdenum increases chemosensitivity of ECA-109 cells to cisplatin and cisplatininduced inhibition of p75NTR expression in esophageal stem cells

AIM:To investigate the effect of molybdenum on the chemosensitivity of esophageal cancer ECA-109 cells to cisplatin and expression of p75NTR in esophageal stem cells.METHODS:ECA-109 cells were divided into four groups:blank control group,cisplatin group,molybdenum group,cisplatin plus molybdenum group.Cell proliferation was measured by methyl thiazolyl tetrazolium(MTT) assay.The expression of p75NTR in human esophageal stem cells was examined by flow cytometry.RESULTS:Cisplatin reduced the percentage of p75NTR-positive cells and the proliferation of ECA-109 cells in a dose-dependent manner.Treatment with molybdenum alone had no significant influence on the proliferation of ECA-109 cells and the percentage of p75NTRpositive cells.However,treatment with molybdenum significantly enhanced the chemosensitivity of esophageal cancer ECA-109 cells to cisplatin and cisplatin-induced inhibition of p75NTR expression in esophageal stem cells when compared to cells incubated with cisplatin alone or blank control cells(all P < 0.05).CONCLUSION:Molybdenum can increase the inhibitory effect of cisplatin on the proliferation of ECA-109 cells,which provides an experimental basis for the use of molybdenum as adjuvant chemotherapeutic agent for esophageal cancer.