三氯乙烯暴露致小鼠肝功能损害与Treg细胞的关系

目的检测分析TCE暴露小鼠肝功能、肝脏Treg细胞分泌的细胞因子IL-10含量以及脾脏Treg细胞数量的变化,探讨Treg细胞与TCE暴露所致肝功能损害的关系。方法雌性BALB/c小鼠饮水摄入2.5 mg/ml和5 mg/ml的TCE,在2、4、8、12周时采集外周血用全自动生化仪测定肝功能,ELISA检测肝组织匀浆IL-10含量,流式细胞仪检测脾脏Treg细胞数量。结果与对照组相比,TCE染毒组小鼠AST和ALT水平明显升高,2周和4周时差异有统计学意义(P<0.05),肝组织匀浆中IL-10含量则明显降低(4周时最低);脾脏Treg细胞数量也明显降低,2、4、8周时差异均有统计学意义(P<0.05);相关分析显示肝功能与肝组织中IL-10含量和Treg细胞数量均呈负相关,且有统计学意义(P<0.05)。结论 TCE暴露引起的肝脏损伤与体内Treg细胞数量减少或功能受损相关。

Relationship between trichloroethylene exposure induced liver damage and Treg cell in mice

Objective To explore the relationships between Treg cell and trichloroethylene exposure induced liver damage through detecting liver function,IL-10 content secreted by hepatic Treg cell and number of spleen Treg cell in mice.Methods Female BALB/c mice were randomly devided into exposed groups that were given 2.5 mg/ml or 5 mg/ml of TCE(10%DMSO solved)contained drinking water,blank group and solvent control group that were only given water or 10%DMSO.At the end of 2,4,8 or 12 weeks of exposure,take the blood samples for liver function examination,and the samples of liver and spleen were also taken for detecting IL-10 content in liver by ELISA,and Treg cell number in spleen by flow cytometry.Results Compared with control group,levels of AST and ALT in TCE treated groups were all significantly increased at 2 weeks and 4 weeks(P<0.05),liver IL-10 content decreased;and spleen Treg cell number was also significantly decreased at 2,4,8 weeks(P<0.05).Correlation analysis showed that liver function negatively correlated with hepatic IL-10 level and number of spleen Treg cells(P<0.05).Conclusion These results suggested that liver damage induced by TCE exposure is well correlated to the function of hepatic Treg cell and the number of spleen Treg cell.