姜黄素对K562细胞STAT5信号分子的影响

目的　了解姜黄素对信号分子的影响 ,探讨其抗白血病的分子机制。方法　用MTT比色法检测细胞增殖活性。用原位杂交法检测细胞STAT5mRNA的表达。用免疫印迹法 (Westernblot ting)检测细胞STAT5蛋白的表达。结果　①姜黄素抑制K5 6 2细胞增殖与作用时间及姜黄素浓度分别呈依赖关系 ,最适作用时间为 12h ,最适作用浓度为 2 5 μmol L。②姜黄素组K5 6 2细胞STAT5mRNA表达的阳性率为 19% ,与K5 6 2细胞组 (31% )相比明显减少 (P <0 .0 1)。③姜黄素组K5 6 2细胞STAT5蛋白的表达 (A值为 15 2 6 6± 76 9)与K5 6 2细胞组 (A值为 2 5 781± 12 4 0 )相比明显减低 (P <0 .0 1)。结论　姜黄素能抑制K5 6 2细胞STAT5信号分子的活化及表达 ,进一步抑制白血病细胞的增殖。

Effect of curcumin on STAT5 signaling molecule in K562 cells

OBJECTIVE: To investigate the effect of curcumin on STAT 5 signaling molecule in K562 cells and its molecular mechanism of antileukemia. METHODS: Cell proliferation was studied by tetrazolium dye assay. The expressions of STAT5 mRNA and protein were assayed by in situ hybridization, and Western blotting respectively. RESULTS: Curcumin could inhibit K562 cell proliferation in a time- and dose-dependent manner. The percentage of STAT5-positive cells was 19% in curcunin group, significantly less than 31% of that in K562 cell group (P < 0.01). The A value of the expression level of STAT5 protein in curcumin group was 15 266 +/- 769, significantly less than 25 781 +/- 1240 of that in K562 cell group (P < 0.01). CONCLUSION: The expressions of STAT5 mRNA and protein in K562 cells were inhibited by curcumin and curcumin could inhibit K562 cell proliferation.