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Enhanced drug delivery to melanoma cells using PMPC-PDPA polymersomes |
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| Authors: | Carla Pegoraro Denis Cecchin Lorena Simon Gracia Nicholas Warren Jeppe Madsen Steven P. Armes Andrew Lewis Sheila MacNeil Giuseppe Battaglia |
| Affiliation: | 1. The Krebs Institute, University of Sheffield, Sheffield, UK;2. The Centre of Membrane Interaction and Dynamics, University of Sheffield, Sheffield, UK;3. The CRUK/YCR Sheffield Cancer Research Centre, University of Sheffield, Sheffield, UK;4. Department of Biomedical Sciences, University of Sheffield, Sheffield, UK;5. Department of Materials Science and Engineering, University of Sheffield, Sheffield, UK;6. Kroto Research Institute, University of Sheffield, Sheffield, UK;g Department of Chemistry, University of Sheffield, Sheffield, UK;h Institute for Research in Biomedicine, University of Barcelona, Barcelona, Spain;i Biocompatibles UK Ltd., Farnham, UK |
| Abstract: | We present the efficient and stable encapsulation of doxorubicin within pH sensitive polymeric vesicles (polymersomes) for intracellular and nuclear delivery to melanoma cells. We demonstrate that PMPC25-PDPA70 polymersomes can encapsulate doxorubicin for long periods of time without significant drug release. We demonstrate that empty polymersomes are non-toxic and that they are quickly and more efficiently internalised by melanoma cells compared to healthy cells. Encapsulated doxorubicin has a strong cytotoxic effect on both healthy and cancerous cells, but when encapsulated it had a preferential effect on melanoma cells indicating that this formulation can be used to achieve an enhanced drug delivery to cancerous cells rather than to the healthy surrounding cells. |
| Keywords: | Polymersomes Melanoma Targeted delivery Doxorubicin |
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