UPLC-MS/MS和HPLC-UV测定肾移植患者麦考酚酸血药浓度的相关性研究

 目的 采用UPLC-MS/MS和HPLC-UV测定肾移植患者麦考酚酸（MPA）血药浓度,考察方法间的相关性, 建立普遍适用的测定结果换算关系。方法 分别采用UPLC-MS/MS测定肾移植患者全血及血浆的麦考酚酸浓度,HPLC-UV测定肾移植患者血浆的麦考酚酸浓度,测定结果采用LEVENE和LSD法进行方差分析, 用Spearman法进行相关性比较。结果 经过统计分析得到3种方法互换方程式,分别为：Y_质谱血浆=1.612 X_质谱全血＋0.686;Y_液相血浆=1.414X_{质谱全血测定}-0.206;Y_液相血浆=0.848 X_质谱血浆-0.692。结论 UPLC-MS/MS测定肾移植患者全血及血浆麦考酚酸浓度与HPLC-UV测定肾移植患者血浆的麦考酚酸浓度,3种方法浓度结果间具有良好的相关性, 通过方程式可进行结果互算。

A Correlational Study of Three Determination Methods of Mycophenolic Acid Blood Concentrations in Renal Transplant Patients

Objective To analyze the correlation between UPLC-MS/MS method and HPLC-UV method for determination of mycophenolic acid (MPA) blood concentrations in renal transplant patients and to establish a generally applicable conversion method of the determination results. METHODS MPA concentrations in whole blood and plasma of transplant patients were determined by UPLC-MS/MS method. Plasma concentrations of MPA were determined by HPLC-UV. Variance analysis of the measure results was done by LEVENE and LSD methods,and the correlation between the methods was analyzed by Spearman method. RESULTS Three conversion formula were obtained as follows: Y_{UPLC-MS/MS plasma}=1.612 X_{UPLC-MS/MS whole blood}+0.686,Y_{HPLC-UV plasma}=1.414X_{UPLC-MS/MS whole blood}-0.206,and Y_{HPLC-UV plasma}=0.848 X_{UPLC-MS/MS whole blood}-0.692. CONCLUSION There is good correlation between UPLC-MS/MS method and HPLC-UV method for the determination of MPA concentration in whole blood and plasma in renal transplant patients. The results can be transformed by equations.