克癃胶囊对大鼠前列腺增生及组织Ki-67、碱性成纤维细胞生长因子和血管内皮生长因子mRNA表达的影响

 目的 探讨克癃胶囊对肾虚血瘀证大鼠前列腺增生的影响及其对前列腺增生相关蛋白、相关细胞因子的影响。方法 建立前列腺增生肾虚血瘀证大鼠模型,观察空白对照组(A组),模型组(B组),非那雄胺组(C组),低、中、高剂量克癃给药组(D、E、F组)大鼠前列腺和前列腺相关因子的变化。结果 ①C~F组大鼠前列腺湿重、前列腺指数均显著低于B组(P<0.05)。②与B组比较,C、E、F组前列腺组织中Ki-67的积分光密度明显降低（P<0.01）,D组无显著性差异(P>0.05)。③与B组比较,C、D、E、F组前列腺组织中碱性成纤维细胞生长因子的积分光密度明显降低（P<0.01）;但D、E、F组前列腺组织中碱性成纤维细胞生长因子的积分光密度均高于C组(P<0.05)。④与B组比较,C、D、E、F组前列腺组织中血管内皮生长因子mRNA的表达降低(P<0.05);但D、E、F组前列腺组织中血管内皮生长因子mRNA的表达均较C组为高(P<0.05)。结论 克癃胶囊可显著抑制大鼠前列腺增生,且呈剂量依赖性。可能与抑制前列腺增生相关蛋白及相关细胞因子的增高及表达密切相关,从而起到抑制前列腺增生的作用。

Effect of Kelong Capsule on Ki-67, bFGF and VEGF mRNA Expression in Benign Prostate Hyperplasia Tissues from Rats

Objective To study the effect of Kelong capsule on kidney deficiency and blood stasis rats prostatic hyperplasia and the prostatic hyperplasia related protein and cell factors. METHODS Establish rat model for benign prostate hyperplasia with kidney deficiency and blood stasis syndrome and observe rats prostate and prostate correlation factors of blank control (A),model (B),the finateride (C),high,medium and low dose kelong (D,E,F). RESULTS ①The prostate wet weight and prostate index of rats in groups C-F were significantly lower than that in group B (P<0.05). ②Compared with group B,Ki-67 integral optical density in prostate tissue obviously decreased in group C,E,F (P<0.01),group D has no significant difference (P>0.05). ③Compared with group B,bFGF integral optical density in prostate tissue in group C,D,E and F decreased obviously (P<0.01)but compared with group C,those in group D,E and F were significantly increased (P<0.05). ④Compared with group B,VEGF mRNA expression in prostate tissue in group C,D,E and F were reduced (P<0.05)； but those in group D,E and F were more than in C group (P<0.05). CONCLUSION Kelong capsule can dose-dependently inhibit prostatic hyperplasia in rats possibly through the inhibition of prostatic hyperplasia related protein and cell factors.