肝细胞癌中Bag-1、血管内皮生长因子的表达及其与预后的关系

 目的　检测Bag-1、血管内皮生长因子（VEGF）分别在肝细胞癌（简称：肝癌）组织、癌旁肝组织及正常肝组织中的表达，探讨它们与肝癌及其预后的关系。方法　采用免疫组织化学SP法检测Bag-1和VEGF在60例肝癌患者癌标本、30例癌旁肝组织、14例正常肝组织标本中的表达，并对其临床病理特点进行分析。结果　Bag-1、VEGF的表达率在肝癌组织中最高，分别为80.0 %（48／60）及73.3 %（44／60），并且与癌旁肝组织[46.7 %（14／30）、43.3 %（13／30）]、正常肝组织[28.6 %（4／14）、21.4 %（3／14）]比较，差异有统计学意义（P＜0.05）。Bag-1、VEGF的表达与肝癌的分期、有无淋巴结转移、有无癌栓形成、有无包膜形成有相关性（P＜0.05），而与患者性别、年龄、肿瘤分级、肿瘤大小及甲胎蛋白（AFP）水平等因素无相关性（P＞0.05）。Bag-1和VEGF在肝癌标本中同时阳性40例，同时阴性8例，二者在肝癌组织中的表达呈正相关（P＜0.05）。Bag-1、VEGF阳性表达组患者1、2年生存率低于阴性组患者，阳性组患者的生存时间明显低于阴性组患者（P＜0.05）。结论　Bag-1、VEGF与肝癌生物学特性密切相关，和肝癌患者预后密切相关。

Expressions of Bag-1 and VEGF in hepatocellular cancer and their relationship with prognosis

Objective　To detect the Bag-1 and VEGF expressions in HCC tissues, cancerous liver tissues and normal liver tissues, and to explore their relationship with prognosis. Methods　Immunohistochemical staining (SP method) was used to detect Bag-1 and VEGF in 60 cases of hepatocellular carcinoma specimens, next to the 30 cases of hepatocellular carcinoma in liver tissue, and 14 cases of normal liver tissue specimens, and their clinicopathological features were analyzed. Results　Bag-1, VEGF expression rates were highest in HCC tissues [80.0 % (48/60), 73.3 % (44/60)] and with adjacent tissues [46.7 % (14/30), 43.3 % (13/30)], normal liver tissue [28.6 % (4/14), 21.4 % (3/14)] expression rates had differences (P < 0.05). Bag-1, VEGF expression had relationship with liver cancer staging, lymph node metastasis, tumor thrombosis formation, the correlation between the presence or absence of capsule formation (P < 0.05), while had no relationship with the patient gender, age, tumor grade, tumor size, and alpha-fetoprotein (AFP) and other factors correlation (P > 0.05). Bag-1 and VEGF expression in HCC specimens positive 40 cases, while negative eight cases, both in HCC tissues expression was positively correlated (P < 0.05). Bag-1, VEGF positive expression in patients 1-year, 2-year survival rates were less than negative patients, the survival time of patients with positive group was significantly lower than the negative patients (P < 0.05). Conclusion　Bag-1 and VEGF expressions are closely related with biological characteristics of liver cancer, and which is closely related to the prognosis of patients with hepatocellular.