Livin影响水疱口炎病毒诱导肺癌A549细胞凋亡效应的实验观察

 目的　探讨Livin表达影响水疱口炎病毒（VSV）对肺癌A549细胞的促凋亡效应。方法　利用RNA干扰技术下调Livin在肿瘤细胞中的表达，通过Tunel染色观察VSV对肺癌A549细胞的凋亡效应，Western blot法检测Livin蛋白的表达，荧光定量法检测Caspase-3的活性。结果　VSV（感染复数＝ 0.1 pfu／细胞）对A549细胞有促凋亡作用；抑制Livin的表达增强了VSV对A549细胞的促凋亡作用，增强了Caspase-3的活性。结论　Livin表达沉默后可能通过影响Caspase-3活性而增强了VSV对A549细胞的促凋亡效应。

Observation on effect of Livin on VSV-induced apoptosis of A549 cells

Objective　To investigate the effect of Livin expression on VSV-induced apoptsis of A549 cells. Methods　The expression of Livin of A549 cells was inhibited by RNA interference. VSV-induced apoptosis of A549 cells was observed by Tunel assay. Protein Level of livin was detected by Western blot. Caspase-3 activity was detected by the fluorescence-based quantitative method. Results　Livin downregulation VSV-induced apoptosis of A549 cells. Inhibited the expression of Livin of A549 cells had increased Caspase-3 activity. Conclusion　The effect of Livin on VSV-induced apoptotic of A549 cells could be increased by RNA interference.