吴茱萸对家兔离体胸主动脉作用机制的实验研究

 目的 观察贵州余庆种植的吴茱萸对家兔离体胸主动脉的作用,探讨其可能的作用机制。方法 取家兔离体胸主动脉条,分为内皮完整组和去内皮组,观察累积质量浓度的吴茱萸水煎液对胸主动脉条的作用。同时用左旋硝基精氨酸甲酯、吲哚美辛苦及BaCl₂预孵育血管环,观察吴茱萸的作用。结果 贵州余庆种植的吴茱萸水煎液能够浓度依赖性地提高血管环张力,且对内皮完整血管环的收缩作用明显强于去内皮血管环。用左旋硝基精氨酸甲酯和吲哚美辛苦预孵育血管环后,吴茱萸使血管环张力上升的幅度降低;用BaCl₂预孵育血管环后对吴茱萸的缩血管作用没有影响。 结论 贵州余庆种植的吴茱萸水煎液对家兔离体胸主动脉有内皮依赖性的收缩作用,同时推测吴茱萸水煎液内还存在可诱导血管内皮细胞释放NO和前列环素的成分,使血管舒张,部分抵消其收缩作用。

Effect of Evodia rutaecarpa Cultivated in Guizhou Yuqing on Rabbit Thoracic Aorta in Vitro and Its Mechanism

OBJECTIVE To investigate the effect of Evodia rutaecarpa cultivated in Guizhou Yuqing on rabbit thoracic aorta in vitro and its possible mechanism. METHODS Evodia rutaecarpa water decoction at concentrations from 0.1 to 1.6 mg·mL^-1 was cumulatively added into organ bath, the isometric tensions of rabbit thoracic aortic rings with intact endothelium or denuded endothelium were recorded, then the thoracic aortic rings were pre-incubated with L-NAME, indometacin and barium chloride respectively to observe the effec of Evodia rutaecarpa. RESULTS The water decoction of Evodia rutaecarpa cultivated in Guizhou Yuqing could dose-dependently increase the tension of rabbit thoracic aortic rings, and the constrictive effect was stronger on endothelium-intact aortic rings than on endothelium-denuded aortic rings. The contraction induced by Evodia rutaecarpa on endothelium-intact aortic rings was decreased by pre-incubation with L-NAME and indometacin. However, Evodia rutaecarpa had no significant effect on endothelium-intact aortic rings when pre-incubated with barium chloride. CONCLUSION The water decoction of Evodia rutaecarpa cultivated in Guizhou Yuqing has endothelium-dependent contracting effect on rabbit thoracic aorta, and it may also stimulate the release of NO and PGI₂ from endothelium cells, which partly compromises its vasoconstrictive effects.