松木层孔菌(Phellinus pini)胞外多糖结构解析

 目的 研究松木层孔菌胞外多糖（PPE）的结构性质。方法 采用高效液相色谱法鉴定纯度并测定相对分子质量;气相色谱、红外光谱、完全酸水解、高碘酸氧化及Smith降解、甲基化反应及其产物的GC-MS联机分析、¹³C-核磁共振对松木层孔菌胞外多糖进行结构性质研究。结果 松木层孔菌胞外多糖为均一组分,多糖含量为92.84%,相对分子质量为3.9×10⁴,由甘露糖、葡萄糖、半乳糖组成,其摩尔比为38.4∶1.76∶1,由α型糖苷键构成,主链部分由α-(1→2)-Man构成,在O-6处形成分支点,支链部分由α-(1→3)-Man和α-(1→6)-Man构成,Man构成松木层孔菌胞外多糖的末端。结论 首次对松木层孔菌胞外多糖的化学结构进行深入的研究。

Structure Characterization of Extracellular Polysaccharide of Phellinus pini

OBJECTIVE To characterize the structure of extracellular polysaccharide of Phellinus pini. METHODS The purity and molecular weight of polysaccharide were determined by HPLC, GC, IR, complete acid hydrolysis, periodate oxidation, Smith degradation, methylation analysis and ¹³C-NMR were applied to determine the structural features. RESULTS Extracellular polysaccharide of Phellinus pini（PPE）had uniform component, and the content of polysaccharide was 92.84%. The molecular weight was 3.9×10⁴. PPE was composed of Man, Glc and Gal with the molar ratio of 38.4∶1.76∶1. The backbone of PPE was composed of α-(1→2)-Man. The branches were located at O-6 of α-(1→2)-Man. The branched chain was consisted of α-(1→3)-Man and α-(1→6)-Man. The terminal sugar was Man. CONCLUSION This is the first in-depth study on the characterization of extracelular polysaccharide of Phelinus pini.