大肠埃希菌噬菌体的分离鉴定及其生物学特性研究 |
| |
引用本文: | 孙荣华,;李菁华,;史红艳,;孙延波,;关显智,;徐德启. 大肠埃希菌噬菌体的分离鉴定及其生物学特性研究[J]. 中国寄生虫病防治杂志, 2009, 0(2): 88-90 |
| |
作者姓名: | 孙荣华, 李菁华, 史红艳, 孙延波, 关显智, 徐德启 |
| |
作者单位: | [1]吉林大学白求恩医学院病原生物学教研室,吉林长春130021; [2]美国食品药品管理局肠道和性传播疾病实验室,吉林长春130021; |
| |
基金项目: | 吉林大学科技创新资助项目(No.2007C71277). |
| |
摘 要: | 目的通过分离鉴定大肠埃希菌噬菌体,研究其生物学特性。方法利用噬菌斑法从环境污水中分离和鉴定大肠埃希菌噬菌体,采用负染法电镜观察噬菌体的形态和大小;提取噬菌体的基因组并进行酶切电泳分析,测定噬菌体感染复数并观察其一步生长曲线。结果通过噬菌斑法分离出9株大肠埃希菌噬菌体。电镜显示,噬菌体头部呈立体对称,有一长尾。琼脂糖凝胶电泳显示,噬菌体基因组大小约30kb。生长曲线表明噬菌体感染宿主菌的潜伏期为23min,爆发时间为39min,裂解量为78。结论9株大肠埃希菌噬菌体中有6株具有较广的噬菌谱,潜伏期短,裂解量明显,为深入研究大肠埃希菌噬菌体的生物学特性及其功能提供了依据。
|
关 键 词: | 大肠埃希菌 噬菌体 感染复数 生物学特征 |
Identification and biological properties of Escherichia coli phages isolated from raw sewage |
| |
Affiliation: | SUN Rong-hua ,LI Jing-hua , SHI Hong-yan , SUN Yan-bo , Guan Xian-zhi , XU De-qi (1. Department of Microbiology, Norman Bethune School of Medicine, J ilin University, Changchun 130021, China; 2. Laboratory of Enteric and Sexually Transmitted Diseases, Food and Drug Administration , USA ) |
| |
Abstract: | Objective To investigate biological properties of phages, Escherichia colt phages were isolated from raw sewage. Methods Bacteriophages were identified using plaque methods, the electron micrographics of phages was carried out to observe the morphology and size of E. colt phage by negative-staining. The extraction of phage genome and the electrophoresis of DNA were performed. Multiplicity of infection and growth curve of phages were determined. Results Nine strains of E. colt phages were identified. Electron micrographics showed that E. colt phages exhibit a cubic symmetry, and these phages have a long tail. Electrophoresis of DNA demonstrated that the size of phage genomes was around 30 kb. The growth curve showed that the incubation period was 23 min, the time of burst phase was 39 min, and the burst size was 78. Conclusion Six strains of phages have a abroad host range in E. coli, a shorter incubation period, and a apparent burst size. These data enable us to explore more properties of E. colt. |
| |
Keywords: | Escherichia colt phages multiplicity of infection biological properties |
本文献已被 维普 等数据库收录! |
|