| 一种改良的按蚊幼虫神经细胞染色体制作方法的研究 |
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| 引用本文： | 龙振昼,;王学忠. 一种改良的按蚊幼虫神经细胞染色体制作方法的研究[J]. 中国寄生虫病防治杂志, 2009, 0(2): 119-120 |
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| 作者姓名： | 龙振昼, 王学忠 |
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| 作者单位： | [1]云南省大理学院病原与媒介生物研究所,云南大理671000; [2]云南省寄生虫病防治所,云南普洱665000 |
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| 摘 要： | 目的研究一种更好的按蚊染色体制作方法,能展示出更清晰而利于分析的染色体组图像。方法选择活跃的处于三龄晚期和（或）四龄早期的按蚊幼虫,用秋水仙碱溶液在室温下培养2h～3h,然后按照一定的规则逐步操作,所获得的染色体标本用作进一步的分析。结果运用这种改良方法制作的按蚊染色体标本,其染色体有丝分裂指数,微小按蚊为2.6%,库态按蚊为2.9%。结论本方法简单易行,便于按蚊染色体的研究。
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| 关 键 词： | 染色体制片 按蚊 分类学 幼虫神经细胞 |
A MODIFIED TECHNIQUE FOR THE PREPARATION OF MITOTIC CHROMOSOMES FROM GANGLION CELLS IN ANOPHELINE LARVAL |
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| Affiliation: | LONG Zhen-zhou , WANG Xue-zhong (1. Institute of Pathogens and Vectors, Dali University, Dali 671000, Yunnan, China; 2. Yunnan Institute of Parasitic Disease, Purer 665000, Yunnan, China) |
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| Abstract: | Objective To study a better method for chromosome preparation of Anopheles, which can show more clear figures for analysis of the karyotypes. Methods The late third and/or early fourth active larvae of Anopheles were chosen and pretreated with colchicine solution at room temperature for 2-3 hours. After a series of steps according to some rules, the chromosome preparation was got for further analysis. Results The mitotic indexes of the chromosome preparation were 2.6G in Anopheles rninirnus and 2. 9% in An. culicifacies, respectively. Conclusion The method described in this study is simple and convenient for investigation of chromosome in anopheline mosquitoes. |
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| Keywords: | Chromosome preparation anopheles systematics larval ganglion cells |
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