阴道毛滴虫分泌型抗原电泳分析

目的研究阴道毛滴虫滋养体分泌型抗原，为阴道毛滴虫免疫诊断寻找特异性抗原。方法用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫移印迹（Western blot）方法，对阴道毛滴虫分泌型抗原进行分析。结果SDS-PAGE分离出9条蛋白带，分子质量单位为15.8-91.2ku，其中主带5条；Western blot显示7条带，在17.0和37.2ku处有2条带缺失。结论阴道毛滴虫分泌型抗原9条蛋白带中，有7条蛋白带特异性免疫反应较强，有望成为阴道毛滴虫免疫诊断的候选抗原。

Electrophoretic analysis of secretive antigens of Trichomonas vaginalis

Objective To study secretive antigens of Trichomonas vaginalis and to find specific antigens for immunological examine of T. vaginalis. Methods The secretive antigens of T. vaginalis from a patient suffering from trichomonad vaginitis were studied by means of SDS-PAGE and Western blot. Results A total of 9 distinct protein bands with MWs 15.8-91.2 ku respectively were demonstrated by means of SDS-PAGE, and 5 of them were main bands. By western blot, the specific anti T. vaginalis antibodies raised in rabbit recognized 7 out of 9 protein bands. Conclusion 7 protein bands out of 9 secretive antigens bands of the parasite showed high immunogenicity, they showed promise as specific antigens for immunological examine of T. vaginalis.