| DMEM体外培养结肠小袋纤毛虫的研究 |
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| 引用本文： | 王天奇,闫文朝,丁轲,张玲,韩利方.DMEM体外培养结肠小袋纤毛虫的研究[J].中国寄生虫病防治杂志,2009(12):907-909. |
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| 作者姓名： | 王天奇 闫文朝 丁轲 张玲 韩利方 |
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| 作者单位： | 河南科技大学动物科技学院,河南洛阳471003 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目（No.2009B23003）. |
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| 摘 要： | 目的建立结肠小袋纤毛虫DMEM培养方法,为进一步研究其生活史及致病机制奠定基础。方法用DMEM培养基培养结肠小袋纤毛虫,观察不同淀粉量、不同血清量、不同初始pH值及不同接种量下的培养效果。结果用DMEM培养基培养结肠小袋纤毛虫,最适淀粉含量为20 mg/安瓿,最适血清含量20%,最适pH值为7.0;种群自然增长率和种群所达到的最大种群密度随接种量的不同而明显改变。结论DMEM培养基可用于结肠小袋纤毛虫的体外培养。
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| 关 键 词： | 结肠小袋纤毛虫 DMEM培养基 种群自然增长率 体外培养 |
Study on the culture of Balantidium coli in DMEM medium in vitro |
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| WANG Tian-qi,YAN Wen-chao,DING Ke,ZHANG Ling,HAN Li-fang.Study on the culture of Balantidium coli in DMEM medium in vitro[J].Chinese Journal of Parasitic Disease Control,2009(12):907-909. |
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| Authors: | WANG Tian-qi YAN Wen-chao DING Ke ZHANG Ling HAN Li-fang |
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| Institution: | (Livestock Science and Technology College, Henan University of Science and Technology, Luoyang 471003, Henan, China) |
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| Abstract: | Objective Development of the vitro culture of Balantidium coli in DMEM medium is crucial to the further study of the life cycle and pathogenetic mechanism of this intestinal protozoa.Methods The population growth of B.coli under different culture conditions,including concentrations of starch and serum,and the initial pH value and various dosages of trophozoites in DMEM medium were compared.Results Results indicated that 20 mg/bottle of starch and 20% serum in the DMEM medium were appropriate for culturing B.coli.The optimal initial pH value was 7.0.The natural population growth rate and the maximum population density clearly differed with different seeded doses of B.coli.Conclusion These findings suggest that DMEM medium could be used to culture B.coli. |
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| Keywords: | Balantidium coli DMEM medium natural population growth rate culture in vitro |
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