巴尔通体在厦门市鼠形动物间及各区域间的分布

目的掌握厦门地区鼠形动物巴尔通体流行情况,证实厦门市鼠形动物感染巴尔通体菌属,为控制人巴尔通体流行提供参考依据。方法根据厦门市行政区划和地理状况划片,随机抽取3个不同区作为调查点。在不同生境采用笼日法捕鼠,取血,用细胞培养法分离巴尔通体;PCR扩增细菌gltA基因片段（376 bp）,并进行序列分析,寻查本地巴尔通体属种。结果厦门市以褐家鼠为巴尔通体主要宿主动物（51.21%）,其次为臭鼩鼱（29.09%）、黄胸鼠（16.36%）及小家鼠、黑家鼠和黄毛鼠等（3.33%）。3个区共采集鼠形动物血样330份,培养分离出巴尔通体84株,感染率25.45%,其中以臭鼩鼱感染率最高,为32.29%,黄胸鼠为24.07%、褐家鼠为23.08%;各调查点鼠形动物巴尔通体感染率分别为：湖里22.64%,海沧28.68%,同安24.51%,差异无统计学意义（P〉0.05）。查出的巴尔通体分别为B.elizabethae、B.queenslandensis和B.tribocorum,其中B.tribocorum分为A、B两群,B群仅感染臭鼩鼱。结论厦门市鼠形动物巴尔通体感染普遍,且存在宿主多样性和菌株对宿主的选择性。

Study on the distribution of Bartonella species in rodent hosts from different areas in Xiamen

Objective To investigate the prevalence of Bartonella species and to identify the species of Bartonella in rodent hosts in Xiamen so as to provide a scientific basis for controlling outbreaks of Bartonella species. Methods According to administrative divisions and geographical portions of Xiamen, three study sites were selected by random sampling. Rodents were captured in cages and blood samples were collected. Bartonella strains were isolated by cell culture. Suspected Bartonella strain isolates were confirmed by amplification of 379 bp of the citrate synthase （gtlA） gene with a specific primer by polymerase chain reaction. The PCR products were sequenced and analyzed and to determine Bartonella species in the areas. Results Rattus norvegicus（51.21%） was the main rodent host in Xiamen, followed by Suncus rnurinus （29.09 % ）, Rattus flavipectus （ 16.36 % ）, and other species （e. g. Mus musculus, Rattus rattus, and Rattus losea ） （3.33%）. Bartonella species were isolated from 84 of 330 blood samples. The infection rate was 25.45%. Among rodent hosts, the infection rate of Bartonella was highest in Suncus murinus （32. 29%）, followed by Rattus flavipectus （24. 07%） and Rattusandersoni （23. 08%）. The infection rate of Bartonella was 22.64% in Huli District, 28.68% in Haicang District, and 24. 51% in Tongan District. There was no statistically significant difference in infection rates among different districts （P〉0.05）. Isolates from rodents were clustered in three genotypes （B. elizabethae, B. queenslandensis, and B. tribocorum）. Of the two B. tribocorum genotypes （13. tribocorurnA and B. tribocorumB）, B. tribocorumB was only isolated from Suncus murinus. Conclusion Rodents in Xiamen were widely infected by Bartonella spp. Hosts were varied, and spp. displayed host selectivity.