低温保存阴道毛滴虫的实验观察

利用5%、10%、15%、20%、25%、30%浓度的二甲基亚砜和甘油肝浸汤培养液对临床分离的阴道毛滴虫进行4℃保存观察。二甲基亚砜和甘油两实验组最适保种浓度分别为15%和10%,50%虫体存活率天数分别为29和27 d,最长保存天数分别为31和29 d;而空白对照组仅为5和7 d。结果表明,15%二甲基亚砜或10%甘油肝浸汤培养液4℃冷藏保存阴道毛滴虫可显著延长其保存期。

Experimental observation of cryopreserved Trichomonas vaginalis

Clinical isolates of Trichomonas vaginalis were preserved at 4 ℃ in liver extract medium with glycerol and dimethylsulfoxide（DMSO） at concentrations of 5%, 10%, 15%, 20%, 25%, and 30% and then observed. The optimal concentrations of DMSO and glycerol for cryopreservation of T. vaginalis were 15% and 10%, respectively. The 50% survival rate in days for T. vaginalis was 29 days and 27 days, respectively. The longest survival in days for T. vaginalis was 31 days and 29 days, respectively, and the blank contrast had a survival of only 7 days. Results indicated that the length of T. vaginalis preservation was prolonged significantly by refrigeration at 4 ℃ with a liver extract medium and 15% DMSO or 10% glycerol.