| 不同浓度胎牛血清肝浸汤培养基培养阴道毛滴虫效果观察 |
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| 引用本文： | 李兴武. 不同浓度胎牛血清肝浸汤培养基培养阴道毛滴虫效果观察[J]. 中国寄生虫病防治杂志, 2009, 0(10): 800-800 |
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| 作者姓名： | 李兴武 |
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| 作者单位： | 郑州大学第一附属医院检验科,河南郑州450052 |
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| 摘 要： | 3株阴道毛滴虫用肝浸汤培养48 h,虫体密度分别为0.7×10^5/ml、1.5×10^5/ml和2.0×10^5/ml;用含终浓度为1%、5%、10%的胎牛血清肝浸汤培养48 h,虫株Ⅰ的密度分别为6.0×10^5/ml、640.0×10^5/ml和1 280.0×10^5/ml,虫株Ⅱ分别为16.0×10^5/ml、1 600.0×10^5/ml和2 400.0×10^5/ml,虫株Ⅲ分别为65.0×10^5/ml、840.0×10^5/ml和520.0×10^5/ml。表明肝浸汤添加适量胎牛血清后培养效果良好。
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| 关 键 词： | 肝浸汤培养基 胎牛血清 阴道毛滴虫 培养 |
Observation of Trichomonas vaginalis cultured in liver extract medium with different concentrations of fetal bovine serum |
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| LI Xing-wu. Observation of Trichomonas vaginalis cultured in liver extract medium with different concentrations of fetal bovine serum[J]. Chinese Journal of Parasitic Disease Control, 2009, 0(10): 800-800 |
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| Authors: | LI Xing-wu |
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| Affiliation: | LI Xing-wu (Clnical Laboratory, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China) |
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| Abstract: | Three strains of Trichomonas vaginalis were cultured for 48 hours in liver extract medium, the respective trophozoite density of T. vaginalis was 0.7 ×10^5/ml, 1.5×10^5/ml, and 2.0 ×10^5/ml. Culturing in the medium with fetal bovine serum at final concentrations of 1%, 5%, and 10% resulted in trophozoite density of 6.0×10^5/ml, 640×10^5/ ml, and 1 280.0×10^5/ml for the first strain, 16.0 ×10^5/ml, 1 600.0 ×10^5/ml, and 2 400×10^5/ml for the second strain, and 65.0×10^5/ml, 840.0×10^5/ml, and 520.0×10^5/ml for the third strain. Results indicated that a liver extract medium with the appropriate concentration of fetal bovine serum provides a good culture medium. |
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| Keywords: | Liver extract medium fetal bovine serum Trichomonas vaginalis culture |
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