bFGF促进骨膜细胞成骨性生长的研究

目的研究bFGF对骨膜细胞的成骨性生长的影响。方法手术分离大鼠骨膜，用胰酶消化法分离骨膜细胞进行元代培养稳定传代后，选择第3～5代细胞随机分成两组；细胞同步化处理后，实验组用终浓度10ug／L的bFGF刺激细胞，对照组正常培养，72h后，用[3H]-TDR方法检测细胞的增殖状态，RT—PCR方法和Westernblot方法检测成骨细胞骨钙素基因的表达。结果10ug／L的bFGF处理细胞72h后，[3H]-TDR方法检测显示细胞DNA合成水平明显上调；而成骨细胞骨钙素基因在mRNA水平和蛋白水平的表达也明显上调。结论bFGF能刺激骨膜细胞的成骨性生长。

To study the ossific proliferation induced by bFGF on periosteum cells.

Objective To study the effect of bFGF on periosteum cells. Methods The periosteum cells were cultured from femoral bone periosteum of the SD rats. The 3- 5 passage ceils were selected to the study and were randomly divided into control group and test group, control group was cultured by normal medium, bFGF of 10ug/L was added in test group. After 72h, two groups cells were detected through 3H -TDR method, and osteocalcin gene expression were detected by RT - PCR and Western blot. Results The cell DNA synthesis were obviously increased by 10ug/L of bFGF determined by 3H -TDR, and mRNA level and protein level of osteocalcin gene were also increased detected by RT- PCR and Western blot. Conclusion bFGF could stimulate the periosteum cells ossific proliferation.