细胞免疫激活对杀伤细胞抑制性受体(KIR) P58+细胞的影响

本研究的目的是观察细胞免疫激活对P5 8基因表达的影响 ,探讨免疫激活与抑制的相互调节关系 ,为临床干细胞移植后的移植物抗宿主病 (GVHD)防治提供理论依据。用IL 2 ,膜抗原 (LP)和ConA分别或联合与人外周血单个核细胞培养 72小时 ,流式细胞术分析单个核细胞中总P5 8.1+ ,P5 8.2 + 细胞及CD3+ ,CD4 + ,CD8+ 和CD16 + CD5 6 + 细胞亚群中的P5 8.1+ 和P5 8.2 + 细胞比率变化。结果发现 ,IL 2 ,LP和ConA分别均可使CD3+ ,CD4 + ,CD8+ 和CD16 + CD5 6 +细胞比率增加 (P <0 .0 1) ,IL 2 ,LP和ConA联合与人外周血单个核细胞培养 72小时有协同刺激P5 8基因表达的作用 (P <0 .0 5 )。结论 :IL 2 ,LP和ConA均可在激活细胞免疫的同时刺激P5 8基因表达或P5 8.1+ 和P5 8.2 + 细胞增殖 ,表明P5 8基因的表达受免疫激活因子调控 ,存在激活诱导表达机制。

Effect of Activation of Cellular Immunity on P58 + Cells Expressing Killer-Cell-Inhibitory Receptor Cells

The purpose of this study was to evaluate the effects of cellular immunity activation on P58 + cells expressing killer cell inhibitory receptor (KIR) and their regulatory function on cellular immunity, and provid theoretical data for preventing graft vers host disease(GVHD) in stem cell transplantation therapy. The mononuclear cells from human peripheral blood were incubated with IL 2, Con A and Lipostin (LP) for 72 hours. The KIR expressing cells, P58.1 + and P58.2 + cells, were analyzed by flow cytometry. The results showed that the percentages of CD3 +, CD4 +, CD8 +, CD16 +CD56 +, P58 1 + and P58.2 + cells were greatly increased after treated with IL 2, Con A and LP, separately or in combination, and the percentages of above cells in combined treatment groups were higher than those of single stimulated groups, especially the percentage of cells in the IL 2+LP group was significantly higher than those in IL 2 and LP singly treated groups. In conclusion: IL 2, Con A and LP possess the ability to induce the expression of KIR and stimulate proliferation of P58.1 + and P58.2 + cells while to activate the celluar immunity response, the expression of P58 gene may be regulated by the activation of cellular immunity.