骨髓间充质干细胞联合肝细胞生长因子基因治疗兔肢体缺血

目的探讨联合应用骨髓间充质干细胞(BMSC)和肝细胞生长因子(HGF)基因治疗兔肢体缺血的疗效。方法 32只新西兰兔切除右后肢股浅动脉并结扎股深动脉建立后肢缺血模型,成模后随机均分为空腺病毒对照组(对照组),BMSC细胞治疗组(BMSC组),HGF基因治疗组(HGF组)和联合治疗组(BMSC+HGF组),各组均采用术肢肌肉内原位注射。治疗28 d后通过动脉造影行侧枝血管计数,治疗30 d后用免疫组化和Western blot法检测注射点周围组织中CD31和HGF的表达。结果 BMSC组及HGF组的侧支血管计数与对照组间差异无统计学意义,而BMSC+HGF组侧支血管计数较其余各组明显增加(P<0.05或P<0.01);免疫组化显示,各处理组CD31表达量明显高于对照组(P<0.05或P<0.01),BMSC组和HGF组间CD31表达量无明显差异(P>0.05),但两者均明显低于BMSC+HGF组(均P<0.05);Western blot显示,各处理组HGF表达量均高于对照组(P<0.05或P<0.01),且HGF在BMSC组、HGF组和BMSC+HGF组中的表达量依次增高,差异均有统计学意义(均P<0.05)...

Combination treatment with bone mesenchymal stem cells and HGF gene therapy for limb ischemia of rabbit

Objective To investigate the therapeutic effect of the combination treatment of bone mesenchymal stem cells(BMSC) and hepatocyte growth factor(HGF) gene therapy on limb ischemia of rabbits.Methods Ischemic model of hind limb was induced in the right hind limb of 32 New Zealand rabbits by ligation of the deep femoral artery and complete excision of the superficial femoral artery.After the model was established,the rabbits were equally randomized into empty plasmid treatment group(control group),BMSC treatmen...