| 牛磺酸对大鼠肝缺血再灌注后小肠损伤的保护作用 |
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| 引用本文： | 佟立权|,赵海峰|,尤立光|,李鑫磊|,李宏宇|,张峰.牛磺酸对大鼠肝缺血再灌注后小肠损伤的保护作用[J].中国普通外科杂志,2011,20(8):827-829. |
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| 作者姓名： | 佟立权| 赵海峰| 尤立光| 李鑫磊| 李宏宇| 张峰 |
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| 作者单位： | 哈尔滨医科大学附属第五医院普通外科; |
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| 基金项目： | 黑龙江省自然科学基金资助项目(D2007-34) |
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| 摘 要： | 目的探讨牛磺酸对大鼠肝缺血再灌注(I/R)后小肠损伤的保护作用。方法将大鼠随机分成假手术组,肝I/R组,牛磺酸预处理+肝I/R组;采用阻断肝动脉、门静脉30min后再灌注的方法,制作肝I/R模型。各组于再灌注3,6,24h分别采血,测定二胺氧化酶(DAO)数值,检测小肠功能;同步切取小肠,测定肠道组织中的SOD及MDA含量,评价肠道自由基损伤程度;切片后行苏木素-伊红(HE)染色,观察病理形态学改变;原位末端标记法(TUNEL)测定细胞凋亡;免疫组化法测定caspase-3表达。结果与假手术组比较,肝I/R组SOD水平明显降低(P<0.05),MDA和DAO水平明显升高(P<0.05),小肠病理损伤严重,凋亡指数明显升高(P<0.05),caspase-3阳性率明显增加(P<0.05)。与I/R组同时间点比较,牛磺酸预处理+I/R组各项指标均明显改善(均P<0.05)。结论牛磺酸对肝I/R后小肠损伤具有保护作用。
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| 关 键 词： | 再灌注损伤 肝 小肠/病理学 牛磺酸 |
| 收稿时间： | 2010/5/19 0:00:00 |
| 修稿时间： | 2010/8/16 0:00:00 |
Protective effect of taurine on small intestinal injury induced by hepatic ischemia/reperfusion in rats |
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| TONG Liquan,ZHAO Haifeng,YOU Liguang,LI Xinlei,LI Hongyu,ZHANG Feng.Protective effect of taurine on small intestinal injury induced by hepatic ischemia/reperfusion in rats[J].Chinese Journal of General Surgery,2011,20(8):827-829. |
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| Authors: | TONG Liquan ZHAO Haifeng YOU Liguang LI Xinlei LI Hongyu ZHANG Feng |
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| Institution: | TONG Liquan,ZHAO Haifeng,YOU Liguang,LI Xinlei,LI Hongyu,ZHANG Feng (Department of General Surgery,the Fifth Affiliated Hospital,Harbin Medical University,Daqing,Heilongjiang 163316,China) |
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| Abstract: | Objective To investigate the protective effect of taurine on small intestinal injury induced by liver ischemia/reperfusion in rats. Methods Rats were randomly assigned into sham operation control group,hepatic I/R group and taurine pretreatment plus hepatic I/R group.Hepatic I/R model was produced by occlusion of both hepatic artery and portal vein for 30 min and then followed by reperfusion.The blood samples were collected at 3,6 and 24 h after reperfusion in each group,and the serum levels of diamine oxid... |
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| Keywords: | Reperfusion Injury Liver Small Intestine/pathol Taurine |
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