碳酸酐酶IX与直肠癌发生和转移的关系

目的 探讨缺氧标记物碳酸酐酶Ⅸ(CA-Ⅸ)在直肠癌患者不同部位表达和对直肠癌细胞体外生长的影响,以及在直肠癌发生和转移中的意义.方法 应用Western blot方法检测CA-Ⅸ在正常直肠组织,直肠腺瘤,直肠癌原发癌灶,系膜组织,肝转移组织中的表达；MTT法检测CA抑制剂乙酰唑胺对体外直肠癌细胞生长能力的影响,判断CA-IX作为直肠癌相关蛋白和转移相关因子的意义.结果 直肠癌癌灶CA-IX阳性率(74.2％)明显高于直肠腺瘤(16.7％)(P＜0.01),而正常直肠组织无CA-Ⅸ表达.癌灶和系膜组织中CA-Ⅸ阳性表达与患者性别、肿瘤大小、分化程度、临床分期无关(均P＞0.05).N0组癌灶CA-Ⅸ阳性率(85.5％)高于N1组(66.7％)和N2组(64.5％)(均P＜0.05)；N0组系膜组织CA-Ⅸ阳性率(5.5％)低于N1组(26.2％)和N2组(29.0％)(均P＜0.05).乙酰唑胺能提高不同浓度5-氟尿嘧啶体外对直肠癌细胞杀伤能力(P＜0.05).结论 CA-Ⅸ能促进直肠癌细胞增殖,与直肠癌发生、发展有关,可作为直肠癌相关肿瘤标记物,但不宜作为癌转移相关因子.

Relations of carbonic anhydrase-IX with tumorigenesis and metastasis of rectal cancer

Objective To investigate the expressions of the hypoxia marker carbonic anhydrases-IX(CA-IX) in the relevant tissues of the patients with rectal cancer and its effect on the growth of rectal cancer cells in vitro,as well as its role in the tumorigenesis and metastasis of rectal cancer. Methods The expressions of CA-IX in normal rectal tissues,rectal adenoma,original tumor tissues of rectal cancer,mesorectums and liver metastases were examined by Western blot analysis.The effect of carbonic anhydrases inhibi...