SEPT7基因抑制U251 MG裸鼠皮下荷瘤侵袭的体内实验研究

目的 探讨SEPT7 基因在体内抑制胶质瘤生长和侵袭的作用.方法 建立人脑胶质母细胞瘤U251细胞来源的裸鼠皮下胶质瘤模型,并给予SEPT7治疗,定期测量肿瘤体积变化,检测肿瘤组织中SEPT7以及侵袭相关蛋白MMP2、MMP9、MT1-MMP、TIMP1、TIMP2和整合素α_v β_3的表达.结果 SEPT7 显著抑制肿瘤生长.治疗组肿瘤体积明显小于对照组(P＜0.01).SEPT7 治疗组中,肿瘤组织细胞内SEPT7表达明显上调.MMP2,MMP9,MT1-MMP和整合素α_v β_3的表达下降,而TIMP1、TIMP2 的表达明显升高.结论 SEPT7基因对于胶质瘤的侵袭和生长具有抑制作用.

Experimental study in vivo of the inhibitory effect of SEPI7 gene therapy for U251 MG human glioblastoma xenograft in nude mice

Objective To investigate the growth - inhibitory and anti - invasion effects of SEPT7 gene on U251 MG human glioma cells in nude mice in vivo. Methods Nude mice bearing subcutaneous U251 MG glioma xenograft tumor model was set up and treated with SEPT7 recombinant adenovirus. The general status of the mice was monitored and the tumor volume was measured every three days. After treatment for 4 weeks, all the tumors were removed and the expression of SEPT7 and other invasion - related proteins ( MMP2 ,MMP9 ,MT1 - MMP.TIMP1 .TIMP2) and integrin αvβ3 were examined. Results SEPT7 could inhibit tumor growth. The average tumor volume of SEPT7 treatment group was smaller than those of the control group and empty vector group significantly. In SEPT7 treatment group, the SEPT7 expression was up - regulated. The expression of MMP2 ,MMP9,MT1 - MMP and integrinαvβ3 was lowered, while TIMP1 and TIMP2 was activated. Conclusion SEPT7 gene therapy has inhibitory effect on the invasion and growth of human gliomas.