| 糖皮质激素对糖皮质激素受体α和β mRNA表达的调节作用 |
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| 引用本文： | 刘宇健,宋亮年,卢建. 糖皮质激素对糖皮质激素受体α和β mRNA表达的调节作用[J]. 中国病理生理杂志, 2002, 18(3): 243-246 |
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| 作者姓名： | 刘宇健 宋亮年 卢建 |
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| 作者单位： | 第二军医大学病理生理教研室，上海 200433 |
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| 基金项目： | 国家自然科学基金资助项目 (No .39870 30 4 ) |
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| 摘 要： | 目的：研究糖皮质激素对人类糖皮质激素受体α和β mRNA表达的调节作用。方法：采用定量逆转录-聚合酶链反应（RT-PCR）检测受体mRNA。结果：人骨肉瘤细胞系、卵巢癌细胞系及外周血白细胞中除有糖皮质激素受体α mRNA表达外，还有糖皮质激素受体β mRNA的表达；糖皮质激素对糖皮质受体α和β mRNA的表达均具有明显的降调作用，且具有剂量依赖性和时间依赖性特点。结论：糖皮质激素受体β在人类多种组织细胞中均有表达，而且其表达受糖皮质激素的调节。
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| 关 键 词： | 糖皮质激素类 RNA 信使 骨肉瘤 卵巢肿瘤 |
| 文章编号： | 1000-4718(2002)03-0243-04 |
| 收稿时间： | 2000-10-31 |
| 修稿时间： | 2000-10-31 |
Effects of glucocorticoids on the glucocorticoid receptorα and β mRNA expression |
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| LIU Yu-jian,SONG Liang-nian,LU Jian. Effects of glucocorticoids on the glucocorticoid receptorα and β mRNA expression[J]. Chinese Journal of Pathophysiology, 2002, 18(3): 243-246 |
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| Authors: | LIU Yu-jian SONG Liang-nian LU Jian |
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| Affiliation: | Department of Pathophysiology, the Second Military Medical University, Shanghai 200433, China |
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| Abstract: | AIM: To clarify the effects of glucocorticoids on the messenger RNA expression of glucocorticoid receptor (GRα and GRβ). METHODS: Messenger RNA of GRα and GRβ were determined by quantitive RT-PCR. RESULTS: Besides GRα mRNA, GRβ mRNA is also expressed in human osteosarcoma cell line HOS-8603, human ovarian carcinoma cell line 3AO and human peripheral white blood cells. After administration of dexamethasone, both GRα mRNA and GRβ mRNA were down-regulated in a time-dependent and dose-dependent manner. CONCLUSION: GRβ expresses in various kinds of human tissue cells. The expression of GRβ is regulated by glucocorticoids. |
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| Keywords: | Glucocorticoids RNA messenger Osteosarcoma Ovarian neoplasms |
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