| 利用免疫磁珠法分离环境及食品中产生TDH副溶血性弧菌的研究 |
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| 引用本文： | 张凡非,杉山宽治,西尾智裕,乡田淑明,秋山真人. 利用免疫磁珠法分离环境及食品中产生TDH副溶血性弧菌的研究[J]. 中国卫生监督杂志, 2004, 11(1): 7-9 |
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| 作者姓名： | 张凡非 杉山宽治 西尾智裕 乡田淑明 秋山真人 |
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| 作者单位： | 1. 山西省疾病预防控制中心,山西,太原,030012
2. 日本静冈县环境卫生科学研究所 |
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| 摘 要： | 采用免疫磁珠法分别从 1份海水、 1份海泥和 3份蛤肉中检出了神奈川现象阳性 ,并产生耐热直接溶血毒素 (TDH )的副溶血性弧菌 ,血清型为O3 :K6。该方法与一般的细菌培养分离法相比 ,极大地提高了环境样品及食品中病原性副溶血性弧菌的检出率 ,也为研究TDH阳性副溶血性弧菌在环境中的分布及预防由其引起的食物中毒提供了依据
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| 关 键 词： | 免疫磁珠法 耐热直接溶血毒素 神奈川现象 副溶血性弧菌 |
| 文章编号： | 1007-6131(2004)01-0007-03 |
| 修稿时间： | 2003-08-28 |
Study of isolating Vibro parahaemolyticus producing TDH from environmental materials and food using immunomagnetic separation method |
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| Zhang Fanfei,et al.. Study of isolating Vibro parahaemolyticus producing TDH from environmental materials and food using immunomagnetic separation method[J]. Chinese Journal of Health Inspection, 2004, 11(1): 7-9 |
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| Authors: | Zhang Fanfei et al. |
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| Abstract: | Immunomagnetic separation was used to isolat e Vibrio parahaemolyticus pathogens from environmental materials and food.Kanagawa phenomenon positive V parahaemolyticus strain s,which produce thermostable direct hemolysin(TDH),were separately isolated fr om one seawater sample,one sea sand sample and three calms.The serotype of thes e strains was O3:K6.Immunomagnetic separation hightens the isolation rate of V parahaemolyticus pathogens from environmental materials and foo d,and it provides scientific basis for the study of the distribution of TDH+V parahaemolyticus strains in environment as well as for the preve ntion of V parahaemolyticus food poisoning. |
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| Keywords: | Immunomagnetic separation Thermostable direc t hemolysin Kanagawa phenomenon WTBXVibrio parahaemolyticus |
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