Phospholipase C-labeled anti-human IgG: inhibition by human IgF. |
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Authors: | R Wei S Riebe |
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Affiliation: | Electro-Nucleonics Laboratories Inc., 4809 Auburn Avenue, Bethesda, Maryland 20014, U.S.A. |
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Abstract: | We report here the inhibition of enzyme activity of phospholipase C-anti-human rabbit IgG conjugate complexed with human IgG. Phospholipase C activity was measured by assaying the release of hemoglobin from erythrocytes. The degree of inhibition varies depending on the relative amount of IgG utilized. A water soluble carbodiimide linking yields a conjugate which is inhibited by IgG, while conjugate prepared by glutaraldehyde is not. Immunodiagnostic assays requiring detection of low levels of antigen or antibody may be possible utilizing the phospholipase C-erythrocyte technique. |
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