附子干姜配伍对阿霉素致大鼠慢性心衰的治疗作用

目的 观察附子于姜配伍治疗阿霉素（ADR）致慢性心衰的作用,并探讨其作用机理.方法 采用盐酸ADR腹腔注射方法,建立慢性心力衰竭大鼠模型,将SD大鼠随机分为6组：对照组,模型组,附子高、低剂量组（3.3,1.65 g/kg）,附子干姜（1∶1）高、低剂量组（6.7,3.3 g/kg）.采用酶联免疫吸附法（ELISA）检测大鼠血清中去甲肾上腺素（NE）、内皮素-1（ET-1）和心脏组织中Na^＋-K^＋-ATPase的活性,运用蛋白免疫印迹杂交法（Western Blot）检测心脏组织蛋白Junctophilin-2 （JP2）的蛋白表达.结果 与模型组比较,附子和附子干姜配伍血清中NE及ET-1含量显著降低,心脏组织中Na^＋-K^＋-ATPase含量及JP2的蛋白表达显著升高,其中附子干姜（1∶1）高剂量组作用更显著（P＜0.01）.结论 附子与附子干姜（1∶1）配伍对ADR致慢性心衰的作用,可能与其增加心脏组织Na^＋-K^＋-ATPase活性,上调Junctophilin-2 （JP2）的蛋白表达有关.

Study on the Compatibility of Aconite and Dried Ginger Treating Rats with Adriamycin-induced Chronic Heart Failure

Objective： To study the compatibility of aconite and dried ginger treating rats with adriamycin-in- duced chronic heart failure and to explore its mechanism. Methods： Hydrochloride adriamycin injection was ap- plied to establish an chronic heart failure animal model, SD rats were randomly divided into 6 groups： normal group, model group, aconite high and low dose groups （3.3,1,65 g/kg）, and aconite and dried ginger（ 1：1 ） high and low dose groups（6.7,3.3 g/kg）. The content of NE and ET-1 in rat serum and activity of Na^＋-K^＋-ATPase in car- diac tissue were detected by ELISA.JP2 protein expression in heart tissue was detected by western blot. Results： Compared with model group,the levels of NE and ET-1 in serum significantly decreased while the activity of Na^＋-K^＋-ATPase and expression of Junctophilin-2 （JP2） protein in cardiac tissue significantly increased. Compati- bility of aconite and dried ginger （1：1） had more significant effect on the high dose group. Conclusion： Compati- bility of aconite and dried ginger treating adriamycin--induced chronic heart failure may relate to increasing activ- ity of Na^＋-K^＋-ATPase and enhancing expression of Junctophilin-2（JP2） protein.