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| 黑大豆种皮花色苷体外抗氧化活性研究 | |
| 引用本文： | 徐金瑞,张名位,刘兴华,张瑞芬,孙玲. 黑大豆种皮花色苷体外抗氧化活性研究[J]. 营养学报, 2007, 29(1): 54-57 |
| 作者姓名： | 徐金瑞 张名位 刘兴华 张瑞芬 孙玲 |
| 作者单位： | 1. 广东药学院公共卫生学院,广州,510310;广东省农业科学院生物技术研究所农业部功能食品重点开放实验室,广州,510640 2. 广东省农业科学院生物技术研究所农业部功能食品重点开放实验室,广州,510640 3. 西北农林科技大学食品科学与工程学院,杨凌,712100 |
| 摘 要： | 目的:探讨黑大豆种皮花色苷(BSCA)的体外抗氧化作用。方法:采用化学模拟体系测定其总抗氧化能力、还原力以及清除DPPH.能力;体外动物实验研究了BSCA对37℃孵育后肝匀浆中谷胱甘肽过氧化物酶(GSH-Px)活力、以及37℃孵育后H2O2诱导的脂质过氧化产物MDA的生成和红细胞溶血的影响。结果:BSCA(390ng/ml)的氧自由基清除活性(ORAC)值为4.63U/ml,其总抗氧化能力强于同浓度的维生素C,其还原力与浓度呈显著正相关(P<0.01),对DPPH.具有较强的清除能力,且可增强GSH-Px活力,抑制MDA的产生和红细胞(RBC)溶血。结论:认为BSCA具有较强的抗氧化作用。 |
| 关 键 词： | 黑大豆种皮 花色苷 抗氧化 体外 |
| 文章编号： | 0512-7955（2007）01-0054-04 |
| 修稿时间： | 2006-07-27 |
THE ANTIOXIDANT EFFECT OF ANTHOCYANIN IN SEED COAT OF BLACK SOYBEAN IN VITRO |
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| XU Jin-rui,ZHANG Ming-wei,LIU Xing-hua,ZHANG Rui-fen,SUN Ling. THE ANTIOXIDANT EFFECT OF ANTHOCYANIN IN SEED COAT OF BLACK SOYBEAN IN VITRO[J]. Acta Nutrimenta Sinica, 2007, 29(1): 54-57 | |
| Authors: | XU Jin-rui ZHANG Ming-wei LIU Xing-hua ZHANG Rui-fen SUN Ling |
| Affiliation: | 1 School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310,China;2 Key Laboratory of Functional Food, Ministry of Agriculture, Bio-tech Research Institute, .Guangdong Academy of Agricultural Sciences, Guangzhou 510640;3College of Food Science and Engineering, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China |
| Abstract: | Objective: To investigate the antioxidant effects of black soybean coat anthocyanins(BSCA).Methods: The capacity of total antioxidant and reduction and DPPH.scavenging ability of BSCA were measured by the chemical simulation.The effect of BSCA on GSH-Px activity and MDA production of liver tissues and hemolysis of RBC induced by H2O2 were analyzed in vitro.Results: The oxygen-radical absorbance capacity(ORAC) value of BSCA(390ng/ml) was 4.63U/ml,the positive correlation(P<0.01) existed between reductive capacity and concentration,BSCA could scavenge DPPH.,enhance the activity of GSH-Px,inhibit production of MDA and hemolysis of RBC.Conclusion: BSCA had antioxidant effect in vitro. |
| Keywords: | black soybean coat anthocyanin anti-oxidative effect in vitro |
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