| 转染VMAT2基因的中国仓鼠卵巢细胞对毒物抵抗作用的研究 |
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| 引用本文： | 王铭维,顾平,崔冬生,王彦永,刘力. 转染VMAT2基因的中国仓鼠卵巢细胞对毒物抵抗作用的研究[J]. 神经损伤与功能重建, 2006, 1(1): 31-34 |
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| 作者姓名： | 王铭维 顾平 崔冬生 王彦永 刘力 |
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| 作者单位： | 河北医科大学附属第一医院(华山医院);河北医科大学附属第一医院(华山医院);河北医科大学附属第一医院(华山医院) |
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| 摘 要： | 目的:研究毒物鱼藤酮和1-甲基-4-苯基吡啶离子(MPP+)对野生型及转染囊泡单胺转运蛋白(VMAT2)基因的中国仓鼠卵巢细胞(CHO)的毒性作用。方法:将不同浓度的MPP+、鱼藤酮与野生型CHO细胞(WT-CHO)和转染VMAT2基因的CHO细胞(VMAT2-CHO)共同培养,采用细胞MTT比色法和形态学观察,探讨毒物对细胞的毒性作用,通过激光共聚焦显微镜观察毒物对2种细胞的细胞内钙浓度([Ca2+]i)的影响。结果:VMAT2-CHO在一定时间(72 h)内对MPP+(0.2-2.0 mmol/L)和鱼藤酮(0.05-1.0μmol/L)的毒性有抵抗作用(P<0.05)。结论:VMAT2可以抵抗一定浓度的MPP+和鱼藤酮引起的神经细胞毒性作用。
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| 关 键 词： | 囊泡单胺转运蛋白 CHO 细胞毒性 细胞内钙浓度 |
| 文章编号： | 1001-117X（2006）01-0031-04 |
| 收稿时间： | 2006-01-29 |
| 修稿时间： | 2006-01-29 |
Resistance of the Transgenic CHO Cell to Toxicity |
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| WANG Ming-wei,GU Ping,CUI Dongs-heng,WANG Yan-yong,LIU li. Resistance of the Transgenic CHO Cell to Toxicity[J]. Neural Injury and Functional Reconstruction, 2006, 1(1): 31-34 |
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| Authors: | WANG Ming-wei GU Ping CUI Dongs-heng WANG Yan-yong LIU li |
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| Affiliation: | Department of Neurology, or Brain Ageing and Cognitive Neuroscience Laboratory of Hebei Province, The First Hospital of Hebei Medical University, Shijiazhuang 050031 ,China |
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| Abstract: | Objective:The toxicity to transgenic CHO of vesicular monoamine transporter 2(VMAT_2) and to wild type CHO generated by MPP~+ and Rotenone was assessed.Methods: MTT assay was used to detect cell survival rate of both WT-CHO and VMAT_2-CHO following exposure to MPP~+ and Rotenone.Meanwhile the change of cell morphology was observed.Fluorescence intensity of intracellular Ca~(2+) concentration in the two groups of cells was measured under the Confocal laser microscopy.Results:Transgenic VMAT_2-CHO cells were resistent to the toxicity of MPP~+(the concentration range: 0.2-2.0 mmol/L) and Rotenone(the concentration range: 0.05-(1.0μmol/L)).Conclusion: Transgenic CHO cells with VMAT2 can resist the toxicity caused by Rotenone and MPP~+. |
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| Keywords: | VMAT_2 CHO cell toxicity intracellular Ca~(2+) |
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