| 脂质过氧化对培养心肌细胞的损伤作用 |
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| 引用本文： | 王光武,赵轶卓,王凡,李才,陈宇.脂质过氧化对培养心肌细胞的损伤作用[J].吉林大学学报(医学版),1993(5). |
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| 作者姓名： | 王光武 赵轶卓 王凡 李才 陈宇 |
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| 作者单位： | 白求恩医科大学应用基础医学研究所
(王光武，赵轶卓，王凡，李才)，白求恩医科大学第一临床学院干部病房(陈宇) |
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| 摘 要： | 应用氢过氧化枯烯作用于培养的心肌细胞,观察到心肌细胞及培养液中脂质过氧化物含量增加,细胞膜流动性下降,细胞维生素E含量减少,~3H—TdR掺入率降低,细胞内酶类漏出增加以及细胞超微结构改变。表明氢过氧化枯烯激发和促进了培养心肌细胞的脂质过氧化过程,提示以氢过氧化枯烯作用于培养心肌细胞,可作为估价体外心肌细胞脂质过氧化损伤的模型之一。
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| 关 键 词： | 过氧化氢类/副作用 丙二醛/分析 维生素E/分析 氢过氧化枯烯 肌酸肌酶/分析 羟丁酸脱氢酶类/分析 乳酸脱氢酶类/分析 心肌/超微结构 细胞 培养的 |
Injurious effect of cumene hydroperoxide on cultured cardiac cells |
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| Wang Guangwu.et al..Injurious effect of cumene hydroperoxide on cultured cardiac cells[J].Journal of Jilin University: Med Ed,1993(5). |
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| Authors: | Wang Guangwu |
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| Abstract: | The effect of cumene hydroperoxide (CHPO) on cultured cardiac cells in rats was investigated. The results showed that the concentration of lipid peroxide and leakage of enzymes in the cells increased; and vitamin E content, cell membrane fluidity, and 3H -TdR penetration in the cells decreased with the increase of CHPO concentration. CHPO might induce cell ultrastructural changes. These indicated that CHPO initiated and facilitated lipid peroxidation in the cultured cardiac cells, and suggested that the effects of CHPO on cultured cardiac cells might be served as one of the models to e-valuate the injurious effect of lipid peroxidation on cardiac cells in vitro. |
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| Keywords: | hydrogen peroxide/AD malondialdehyde/AN creatine kinase/AN hydroxybutyrate dehydrogenase/AN LDH/AN myocardium/UL cells cultured cumene hydroperoxide |
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