重组日本血吸虫26kDaGST抗原免疫家兔后抗体动态观察

目的： 探讨重组日本血吸虫２６ ｋ Ｄａ Ｇ Ｓ Ｔ 抗原免疫家兔后抗体水平动态变化。方法： 家兔随机分为免疫组与佐剂对照组，免疫组用纯化重组日本血吸虫 ２６ ｋ Ｄａ Ｇ Ｓ Ｔ 抗原加福氏佐剂免疫；佐剂对照组用０．８５％ 盐水加福氏佐剂免疫。免疫后，每兔逐周取耳静脉血，采用酶联免疫吸附试验（ Ｅ Ｌ Ｉ Ｓ Ａ） 检测血清中特异性抗体水平。结果： 免疫组家兔从免疫后第４ ｗ ｋ 起血清中特异性抗 Ｇ Ｓ Ｔ 抗体升高，且维持在较高的水平达６５ ｗ ｋ。结论： 重组日本血吸虫 ２６ ｋ Ｄａ Ｇ Ｓ Ｔ 抗原免疫家兔后，特异性抗 Ｇ Ｓ Ｔ 抗体水平较对照组明显升高，且至少能维持 １ 年。

OBSERVATION ON THE DYNAMICS OF SPECIFIC ANTI GST ANTIBODIES IN RABBITS IMMUNIZED WITH RECOMBINANT 26 kDa GST OF SCHISTOSOMA JAPONICUM

AIM: To investigate the dynamics of the anti-GST antibodies in rabbits immunized with recombinant 26 kDa GST of Schistosoma japonicum. METHODS: The specific antibodies were detected weekly by GST-ELISA in rabbits vaccinated with purified recombinant 26 kDa GST antigen of S. japonicum plus FCA/IFCA; rabbits immunized with 0.85% saline plus FCA/IFCA served as control group. RESULTS: The specific anti-GST antibodies began to increase at week 4 post-immunization, the mean OD value in rabbits vaccinated with recombinant GST could reach 1.03 +/- 0.46 (adjuvant control group only 0.42 +/- 0.04). The experiment was completed at week 65 post-immunization when the mean OD values for specific anti-GST antibody in immunized rabbits were 0.94 +/- 0.26(0.29 +/- 0.16 in the control group). CONCLUSION: Strong anti-GST antibody responses could be induced in rabbits vaccinated with recombinant 26 kDa GST of S. japonicum.