| 用MTT法进行白血病体外药物敏感性检测的临床意义 |
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| 引用本文： | 陈新峰,杨菁. 用MTT法进行白血病体外药物敏感性检测的临床意义[J]. 福建医科大学学报, 2009, 43(2): 169-171 |
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| 作者姓名： | 陈新峰 杨菁 |
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| 作者单位： | 福建医科大学,附属协和医院药学部,福州,350001 |
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| 摘 要： | 目的探讨使用MTT比色法进行白血病体外药物敏感性检测的可行性．为MTT法作为肿瘤药敏检测技术应用于临床提供理论依据。方法采用MTT比色法测定47例血液病患者外周血和骨髓白血病细胞对14种临床常用化疗药物的敏感性，并与化疗药物的实际疗效作对照。结果47例患者中，体外敏感／体内敏感（S／S）30例、体外敏感／体内耐药（S／R）7例、体外耐药／体内耐药（R／R）9例、体外耐药／体内敏感（R／S）例；体外药敏与体内疗效的总符合率为82．98％，阳性符合率为81.09％，阴性符合率90％。敏感度96．77％，特异度56．25％。结论采用MTT法预测化疗药物的敏感性，临床相关性好。
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| 关 键 词： | 白血病 比色法 氮蓝四唑 药物耐受性 药物筛选试验 抗肿瘤 |
Application of MTT Assay in the in vitro Tumor Chemosensitivity Test among Patients with Leukemia and Its Clinical Significance |
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| CHEN Xinfeng,YANG Qing. Application of MTT Assay in the in vitro Tumor Chemosensitivity Test among Patients with Leukemia and Its Clinical Significance[J]. Journal of Fujian Medical University, 2009, 43(2): 169-171 |
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| Authors: | CHEN Xinfeng YANG Qing |
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| Affiliation: | CHEN Xinfeng,YANG Qing Department of Pharmacy,The Affiliated Union Hospital,Fujian Medical University,Fuzhou 350001,China |
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| Abstract: | Objective To investigate the feasibility of the application of MTT assay in the in vitro leukemia chemosensitivity test,in attempt to provide theoretical basis for its clinic application. Methods Peripheral blood cells and bone marrow leukemia cells collected from 47 patients were detected by MTT colorimetric assay in light of their sensitivity to 14 drugs. The relationships between the findings and the practicalities of chemotherapeutics were analyzed. Results Among 47 patients,30 cases were S/S,7 cases S/... |
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| Keywords: | leukemia colorimery nitroblue tetrazolium drug tolerance drug screening assays antitumor |
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