大肠癌MTS基因突变和p16蛋白表达

目的]分析大肠癌MTS基因突变与p16蛋白表达的关系。方法]应用PCR-SSCP技术及LSAB免疫组织化学法同时检测 135例大肠癌MTS基因的突变和其基因产物(p16蛋白)的表达情 况。结果]135例大肠癌MTS基因的突变率为4.44％，与正常粘 膜组织相比差异并无显著性，p16蛋白阳性率为59.25％，与 正常粘膜组织相比有显著性差异(P<0.05)。结论]大肠癌极少 发生MTS基因突变，但有较高的p16蛋白表达下调。MTS基因表 达障碍可能有另外原因。

The Mutation of MTS Gene and Expression of p16 Protein in Colorectal Cancer

To investigate the mutation of MTS gene and the expression of p16 protein,which may have a great effect on the carcinogensis of colorectum.PCR SSCP technology was used to detected the mutation of MTS gene with a primer of exon 2 of MTS gene,and the expression of its product p16 protein was detected using LSAB immunohistochemical method in 135 cases with colorectal cancer.The mutation rate of MTS gene of 135 cases with colorectal cancer was 4.44%.Although there was no difference between colorectal cancer and normal mucous membranes for mutation rate of MTS gene,it was founded that there was a significant difference in the expression of p16 protein between two groups (the positive rate of p16 was 59.26% in colorectal cancer versus 100% in normal tissues).Conclusion]The mutation of MTS gene in colorectal cancer is not common,however,the expression of p16 protein is much lower.There must be an another regular mechanism controlling p16 protein synthesis which may be one of factors of the carcinogenesis in colorectal cancer.