Regulation of ion channels in rat hepatocytes |
| |
Authors: | S. Breit H.-A. Kolb H. Apfel C. Haberland M. Schmitt D. Häussinger J. Graf F. Lang |
| |
Affiliation: | Physiologisches Institut, Universit?t Tübingen, Gmelinstrasse 5, D–72076 Tübingen, Germany, DE Medizinische Klinik der Universit?t Düsseldorf, Düsseldorf, Germany, DE Institut für Allgemeine und Experimentelle Pathologie, Universit?t Wien, Vienna, Austria, AT
|
| |
Abstract: | Patch-clamp studies have been performed to elucidate single ion channels in rat hepatocytes. In rat hepatocytes two types of ion channel have been identified: an inwardly rectifying K+ channel with a mean inward conductance of 55 ± 6.5 pS (n = 20) and a mean outward conductance of 25 ± 3.2 pS (n = 20) in the inside-out configuration with 145 mmol/l KCl on either side of the patch as well as an outwardly rectifying Cl– channel with a mean outward conductance of 30 ± 4.5 pS (n = 8) and a mean inward conductance of 10 ± 2.3 pS (n = 6) in the inside-out configuration with symmetrical 145 mmol/l KCl. The open probability of these channels is virtually insensitive to Ca2+ activity on the intracellular side. Accordingly, the Ca2+ ionophore ionomycin had no effect on cell membrane potential. Dibutyryl-cAMP (db-cAMP) hyperpolarizes the cell membrane and increases the activity of the 55-pS inwardly rectifying K+ channel by reducing the duration of closure between bursts. Forskolin similarly hyperpolarizes the cell membrane. The inwardly rectifying K+ channel is inhibited by progesterone, while the outwardly rectifying Cl– channel is insensitive to progesterone. Received: 21 May 1997 / Received after revision: 7 August 1997 / Accepted: 19 August 1997 |
| |
Keywords: | Patch clamp Cell membrane potential K+ conductance K+ channels Cl channels cAMP Cell volume Progesterone Hepatocytes |
本文献已被 SpringerLink 等数据库收录! |
|