| 圆叶牵牛毛状根的诱导及培养 |
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| 引用本文： | 李丽英,杨世海,徐大卫,张悦. 圆叶牵牛毛状根的诱导及培养[J]. 中国现代中药, 2011, 13(6): 30-33 |
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| 作者姓名： | 李丽英 杨世海 徐大卫 张悦 |
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| 作者单位： | 1. 吉林农业大学中药材学院,吉林,长春,130118
2. 中国药材公司,北京,100195 |
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| 摘 要： | 目的:利用发根农杆菌,R1601,1000,1025,15834诱导药用植物圆叶牵牛产生毛状根。方法:采用共培养方法,通过对外植体、菌种、浸染时间、预培养、共培养、外源激素等因素对转化率的影响。结果:利用发根农杆菌1601,预培养60h的叶片、叶柄为转化材料,感染6～8min,加入1mg.L-1NAA转化率最高。结论:圆叶牵牛毛状根的诱导成功,为其遗传转化提供了依据。
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| 关 键 词： | 圆叶牵牛 发根农杆菌 毛状根 |
| 收稿时间： | 2011-01-20 |
Induction and Culture of Hairy Roots of Pharbitis purpurea ( L.) Voigt |
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| LI Li-ying,YANG Shi-hai,XU Da-wei and ZHANG Yue. Induction and Culture of Hairy Roots of Pharbitis purpurea ( L.) Voigt[J]. Modern Chinese Medicine, 2011, 13(6): 30-33 |
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| Authors: | LI Li-ying YANG Shi-hai XU Da-wei ZHANG Yue |
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| Affiliation: | LI Li-ying1,YANG Shi-hai1,XU Da-wei2,ZHANG Yue1(1. College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China,2. China National Corp. of Traditional & Herbal Medicine,Beijing 100195,China) |
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| Abstract: | Objective:Hairy root of Pharbitis purpurea(L.)Voigt were induced by the infection of four Agrobacterium rhizogenes strains 1000,1025,15834 and R1601,and an in vitro culture system of the hairy roots was established.Methods:Hairy roots were induced by co-culture.Effects of explants,Agrobacterium rhizogenes,infecting time,pre-culture time,co-culture time and phytohormones on the transformation frequency were studied.Results:The highest induction frequency was obtained from blade leafs and leaf stalk with 60 h... |
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| Keywords: | Pharbitis purpurea(L.)Voigt Agrobacterium rhizogenes Hairy roots |
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