| 肝癌患者C型乙型肝炎病毒前S基因突变研究 |
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| 引用本文： | 强福林,吴徐明,刘继斌,李海波.肝癌患者C型乙型肝炎病毒前S基因突变研究[J].肿瘤基础与临床,2011,24(3):196-198. |
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| 作者姓名： | 强福林 吴徐明 刘继斌 李海波 |
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| 作者单位： | 1. 南通市肿瘤医院,江苏南通,226361
2. 南通市疾病预防控制中心,江苏南通,226007 |
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| 基金项目： | 南通市社会发展科技计划项目 |
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| 摘 要： | 目的探讨肝癌患者C型乙型肝炎病毒(HBV)前S(pre-S)基因的突变情况。方法自肝癌患者血清中提取HBV基因组DNA,扩增其pre-S基因片段并进行序列分析,用MEGA3.1和C lustalx1.8作为所测基因进行分型,并对比pre-S基因序列。结果所测样品中,C型占56.0%,B型占20.0%,B/C混合感染型占24.0%。肝癌患者C型HBV前S区普遍存在基因突变。肝癌患者C型HBV pre-S1区域以个体化的点突变为主,而pre-S2区域则存在大量的缺失突变。结论 Pre-S基因是肝癌患者C型HBV的高变区。
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| 关 键 词： | 乙型肝炎病毒 前S基因 肝癌 |
Mutations of Pre-S Gene of Genotype C Hepatitis B Virus in Patients with Hepatocarcinoma |
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| Qiang Fulin,Wu Xuming,Liu Jibin,Li Haibo.Mutations of Pre-S Gene of Genotype C Hepatitis B Virus in Patients with Hepatocarcinoma[J].journal of basic and clinical oncology,2011,24(3):196-198. |
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| Authors: | Qiang Fulin Wu Xuming Liu Jibin Li Haibo |
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| Institution: | 1.Nantong Tumor Hospital,Nantong 226361,China;2.Nantong Center for Disease Control and Prevention,Nantong 226007,China) |
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| Abstract: | Objective To investigate the mutations of pre-S gene of genotype C hepatitis B virus(HBV) in patients with hepatocarcinoma.Methods The pre-S gene of HBV from patients with hepatocarcinoma was amplified and sequenced.The sequences of all the samples were analyzed by using MEGA3.1 and Clustalx1.8.Results Of all the samples,56.0% were genotype C,20.0% were genotype B,and 24.0% were the B/C mix-infected genotype.Code gene mutation and deletion mutation were popular in pre-S1 and pre-S2 regions.Conclusion Pre-S gene mutation of genotype C HBV may play an important role in HBV-related hepatocarcinogenesis. |
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| Keywords: | hepatitis B virus pre-S gene hepatocarcinoma |
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