|
|||||
|
|
| 激光捕获显微切割纯化的人正常支气管上皮与肺鳞癌组织的定量蛋白质组学研究 | |
| 引用本文： | 许艳,曾谷清,金龙玉,汤参娥,李萃,肖志强. 激光捕获显微切割纯化的人正常支气管上皮与肺鳞癌组织的定量蛋白质组学研究[J]. 国际病理科学与临床杂志, 2011, 31(3): 185-192. DOI: 10.3969/j.issn.1673-2588.2011.03.001 |
| 作者姓名： | 许艳 曾谷清 金龙玉 汤参娥 李萃 肖志强 |
| 作者单位： | 1. 中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙,410008 2. 中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙,410008;南华大学医学院外科学总论教研室,湖南,衡阳,421001 3. 中南大学湘雅三医院胸外科,长沙,410013 |
| 基金项目： | 国家自然科学基金，教育部新世纪优秀人才培养计划基金，湖南省自然科学基金，湖南省卫生厅科研基金，湖南省教育厅科研基金 |
| 摘 要： | 目的:建立肺鳞癌(human lung squamous carcinoma,HLSC)组织与正常支气管上皮(normal human bronchial epithelial,NHBE)组织的差异蛋白质表达谱.方法:运用激光捕获显微切割(laser capture microdissection,LCM)技术从HLS... |
| 关 键 词： | 肺鳞癌 差异蛋白质 激光捕获显微切割 定量蛋白质组学 iTRAQ |
Quantitative proteomic study for purified human lung squamous carcinoma and normal human bronchial epithelial by laser capture microdissection |
|
| XU Yan,ZENG Guqing,JING Longyu,TANG Cane,LI Cui,XIAO Zhiqiang. Quantitative proteomic study for purified human lung squamous carcinoma and normal human bronchial epithelial by laser capture microdissection[J]. Journal of International Pathology and Clinical Medicine, 2011, 31(3): 185-192. DOI: 10.3969/j.issn.1673-2588.2011.03.001 | |
| Authors: | XU Yan ZENG Guqing JING Longyu TANG Cane LI Cui XIAO Zhiqiang |
| Affiliation: | XU Yan1,ZENG Guqing1,JING Longyu2,TANG Can'e1,LI Cui1,XIAO Zhiqiang1(1.Key Laboratory of Cancer Proteomics of Chinese Ministry of Health,Xiangya Hospital,Central South University,Changsha 410008,2.Department of General Introduction to Surgery,School of Medicine,University of South China,Hengyang Hunan 421001,3.Department of Cardiothoracic Surgery,Third Xiangya Hospital,Changsha 410013,China) |
| Abstract: | Objective To establish the differential protein profile for human lung squamous carcinoma(HLSC) and normal human bronchial epithelium(NHBE).Methods Laser capture microdissection(LCM) was used to purify the target cells from HLSC tissues and matched NHBE.A stable isotope labeled strategy using iTRAQ together with 2 dimensional chromatography/hybrid quadrupole time of flight(2D-LC/Q-STAR) mass spectrometry was performed to separate and identify the differential expression proteins.Results A total of 96 differ... |
| Keywords: | iTRAQ |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |